The relationship between knee moments and markers of knee osteoarthritis progression has not been examined in different knee osteoarthritis subtypes. The objective was to examine relationships between external knee moments during gait and tibiofemoral cartilage thickness in patients with nontraumatic and posttraumatic knee osteoarthritis. For this cross-sectional study, participants with knee osteoarthritis were classified into two groups:​ nontraumatic (n = 22;​ mean age 60 years) and posttraumatic (n = 19;​ mean age 56 years, history of anterior cruciate ligament rupture). Gait data were collected with a three-dimensional motion capture system sampled at 100 Hz and force plates sampled at 2000 Hz. External knee moments were calculated using inverse dynamics. Cartilage thickness was determined with magnetic resonance imaging (T1-weighted, 3D sagittal gradient-echo sequence). Linear regression analyses examined relationships between cartilage thickness with knee moments, group, and their interaction. A higher knee adduction moment impulse was negatively associated with medial to lateral cartilage thickness ratio (B = −1.97). This relationship differed between participants in the nontraumatic osteoarthritis group (r = −0.56) and posttraumatic osteoarthritis group (r = −0.30). A higher late stance knee extension moment was associated with greater medial femoral condyle cartilage thickness (B = −0.86) and medial to lateral cartilage thickness (B = −0.73). These relationships also differed between participants in the nontraumatic osteoarthritis group (r = −0.61 and r = −0.51, respectively) and posttraumatic osteoarthritis group (r = 0.10 and r = 0.25, respectively). Clinical Significance:​ The relationship between knee moments with tibiofemoral cartilage thickness differs between patients with nontraumatic and posttraumatic knee osteoarthritis. The potential influence of mechanical knee loading on articular cartilage may also differ between these subtypes.