Some new, simple and efficient stereological methods and their use in pathological research and diagnosis

Gundersen, H. J. G.; Bendtsen, T. F.; Korbo, L.; Marcussen, N.; Møller, A.; Nielsen, K.; Nyengaard, J. R.; Pakkenberg, B.; Sørensen, F. B.; Vesterby, A.; West, M. J.

Affiliations

¹Stereological Research Laboratory, University Institute of Pathology and 2nd University Clinic of Internal Medicine, Institute of Experimental Clinical Research, University of Århus

²Neurological Research Laboratory, Hvidovre Hospital

³Institute of Pathology, University of Copenhagen, Rigshospitalet

⁴University Institute of Pathology, Århus Amtsygehus, Denmark

Abstract and keywords

Stereology is a set of simple and efficient methods for quantitation of three-dimensional microscopic structures which is specifically tuned to provide reliable data from sections. Within the last few years, a number of new methods has been developed which are of special interest to pathologists. Methods for estimating the volume, surface area and length of any structure are described in this review. The principles on which stereology is based and the necessary sampling procedures are described and illustrated with examples. The necessary equipment, the measurements, and the calculations are invariably simple and easy.

Keywords: Cavalieri’s principle; isotropic sections; star volume; surface area; stereology; vertical sections; volume

Links

DOI: 10.1111/j.1699-0463.1988.tb05320.x

PubMed: 3288247

WoS: A1988N950400001

Cited Works (1)

Year	Entry
1984	Parfitt AM. Age-related structural changes in trabecular and cortical bone: cellular mechanisms and biomechanical consequences. Calcif Tiss Int. 1984;36(suppl 1):S123-S128.

Cited By (16)

Year	Entry
2002	Wehrli F, Saha P, Gomberg B, Song H, Snyder P, Benito M, Wright A, Weening R. Role of magnetic resonance for assessing structure and function of trabecular bone. Top Magn Reson Imaging. October 2002;13(5):335-355.
1996	Amling M, Pösl M, Ritzel H, Hahn M, Vogel M, Wening VJ, Delling G. Architecture and distribution of cancellous bone yield vertebral fracture clues: a histomorphometric analysis of the complete spinal column from 40 autopsy specimens. Arch Orthop Trauma Surg. July 1996;115(5):262-269.
1989	Vesterby A, Gundersen H, Melsen F. Star volume of marrow space and trabeculae of the first lumbar vertebra: sampling efficiency and biological variation. Bone. 1989;10(1):7-13.
1990	Vesterby A. Star volume of marrow space and trabeculae in iliac crest: sampling procedure and correlation to star volume of first lumbar vertebra. Bone. 1990;11(3):149-155.
1993	Gundersen HJG, Boyce RW, Nyengaard JR, Odgaard A. The connEulor: unbiased estimation of connectivity using physical disectors under projection. Bone. May–June 1993;14(3):217-222.
2021	Klose-Jensen R, Hartlev LB, Thomsen JS, Nyengaard JR, Boel LWT, Laursen M, Laurberg TB, Stengaard-Pedersen K, Hauge E-M. Calcified cartilage in patients with osteoarthritis of the hip compared to that of healthy subjects: a design-based histological study. Bone. February 2021;143:115660.
2022	Lamarche BA, Thomsen JS, Andreasen CM, Lievers WB, Andersen TL. 2D size of trabecular bone structure units (BSU) correlate more strongly with 3D architectural parameters than age in human vertebrae. Bone. July 2022;160:116399.
1997	Hildebrand T, Rüegsegger P. A new method for the model-independent assessment of thickness in three-dimensional images. J Microsc (Oxford). 1997;185(1):67-75.
1996	Wong M, Wuethrich P, Eggli P, Hunziker E. Zone-specific cell biosynthetic activity in mature bovine articular cartilage: a new method using confocal microscopic stereology and quantitative autoradiography. J Orthop Res. May 1996;14(3):424-432.
2002	Hunziker EB. Articular cartilage repair: basic science and clinical progress. a review of the current status and prospects. Osteoarthritis Cartilage. June 2002;10(6):432-463.
2002	Hunziker EB, Quinn TM, Häuselmann H-J. Quantitative structural organization of normal adult human articular cartilage. Osteoarthritis Cartilage. July 2002;10(7):564-572.
2005	Szafranski JD. Cartilage Mechanobiology: The Effects of Loading on the Fine Structure and Function of Chondroitin Sulfate Glycosaminoglycans [PhD thesis]. Cambridge, MA: Massachusetts Institute of Technology; June 2005.
2001	Tanck EJM. Mechanical Regulation of Bone Development [PhD thesis]. Nijmegen, Netherlands: Katholieke Universiteit Nijmegen; June 2001.
2020	Dreyer CH. Enhance Bone Regeneration and Implant Fixation by Novel Substitute With Combined Angiogenic and Osteogenic Factors in Animal Models [PhD thesis]. University of Southern Denmark (SDU); 2020.
1999	Awad HA. Mesenchymal Stem Cell Seeded Collagen Scaffolds for Tendon Repair [PhD thesis]. University of Cincinnati; 1999.
2010	Melhus G. Molecular Studies on Bone With Focus on Fracture Healing in Experimental Osteoporosis [PhD thesis]. Oslo, Norway: University of Oslo; 2010.