Osteocyte differentiation in the tibia of newborn rabbit: an ultrastructural study of the formation of cytoplasmic processes

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Palumbo, Carla¹; Palazzini, Silvana¹; Zaffe, Davide¹; Marotti, Gastone¹

Osteocyte differentiation in the tibia of newborn rabbit: an ultrastructural study of the formation of cytoplasmic processes

Acta Anat. 1990;137(4):350-358

©1990 Karger AG, Basel

Affiliations

¹Istituto di Anatomia umana normale, Università di Modena, Italia
Abstract and keywords

The morphological changes undergone by the osteoblast at the ultrastructural level, during its differentiation into osteocyte, were studied in the primary parallel-fibred bone of the newborn rabbit by means of incomplete three-dimensional reconstruction from partially serial-sectioned preosteocytes. The findings obtained suggest that the formation of osteocyte cytoplasmic processes is an asynchronous and asymmetrical phenomenon that seems to precede the mineralization of the organic matrix and to give rise to an asymmetrical mature osteocyte. The functions of cytoplasmic processes as regards bone formation, cell nutrition and osteoblast modulation are discussed. The mechanism by which the osteoblast ‘enters’ the bone matrix is hypothesized.

Keywords: Osteocyte differentiation; Osteoblast; Preosteocyte; Cytoplasmic processes; Mineralizing surface; Newborn rabbit
Links

DOI: 10.1159/000146907

PubMed: 2368590

WoS: A1990DD40800010
History

Received: 1989-04-04

Accepted: 1989-07-03

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Cited By (28)

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2004	You L, Weinbaum S, Cowin SC, Schaffler MB. Ultrastructure of the osteocyte process and its pericellular matrix. Anat Rec. June 2004;278A(2):505-513.
2015	Florencio-Silva R, Sasso GRdS, Sasso-Cerri E, Simões MJ, Cerri PS. Biology of bone tissue: structure, function, and factors that influence bone cells. BioMed Res Int. 2015;2015:421746.
1990	Palumbo C, Palazzini S, Marotti G. Morphological study of intercellular junctions during osteocyte differentiation. Bone. 1990;11(6):401-406.
1996	Mullender MG, van der Meer DD, Huiskes R, Lips P. Osteocyte density changes in aging and osteoporosis. Bone. February 1996;18(2):109-113.
2001	Kamioka H, Honjo T, Takano-Yamamoto T. A three-dimensional distribution of osteocyte processes revealed by the combination of confocal laser scanning microscopy and differential interference contrast microscopy. Bone. February 2001;28(2):145-149.
2005	Sugawara Y, Kamioka H, Honjo T, Tezuka K-i, Takano-Yamamoto T. Three-dimensional reconstruction of chick calvarial osteocytes and their cell processes using confocal microscopy. Bone. May 2005;36(5):877-883.
2010	Schneider P, Meier M, Wepf R, Müller R. Towards quantitative 3d imaging of the osteocyte lacuno-canalicular network. Bone. November 2010;47(5):848-858.
2014	Schaffler MB, Cheung W-Y, Majeska R, Kennedy O. Osteocytes: master orchestrators of bone. Calcif Tiss Int. January 2014;94(1):5-24.
2006	Franz-Odendaal TA, Hall BK, Witten PE. Buried alive: how osteoblasts become osteocytes. Dev Dyn. January 2006;235(1):176-190.
1996	Mikuni-Takagaki Y, Suzuki Y, Kawase T, Saito S. Distinct responses of different populations of bone cells to mechanical stress. Endocrinology. May 1996;137(5):2028-2035.
2013	Dallas SL, Prideaux M, Bonewald LF. The osteocyte: an endocrine cell … and more. Endocr Rev. October 2013;34(4):658-690.
2008	Shapiro F. Bone development and its relation to fracture repair: the role of mesenchymal osteoblasts and surface osteoblasts. Eur Cell Mater. 2008;15:53-76.
1990	Marotti G, Cane V, Palazzini S, Palumbo C. Structure-function relationships in the osteocyte. Ital J Min Electrol Metabol. 1990;4(2):93-106.
1997	Kato Y, Windle JJ, Koop BA, Mundy GR, Bonewald LF. Establishment of an osteocyte‐like cell line, MLO‐Y4. J Bone Miner Res. December 1997;12(12):2014-2023.
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2003	Gluhak-Heinrich J, Ye L, Bonewald LF, Feng JQ, MacDougall M, Harris SE, Pavlin D. Mechanical loading stimulates dentin matrix protein 1 (DMP1) expression in osteocytes in vivo. J Bone Miner Res. May 2003;18(5):807-817.
2020	Kegelman CD, Coulombe JC, Jordan KM, Horan DJ, Qin L, Robling AG, Ferguson VL, Bellido TM, Boerckel JD. YAP and TAZ mediate osteocyte perilacunar/canalicular remodeling. J Bone Miner Res. January 2020;35(1):196-210.
1994	Aarden EM, Burger EH, Nijweide PJ. Function of osteocytes in bone. J Cell Biochem. July 1994;55(3):287-299.
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1995	Burger EH, Klein-Nulend J, Van Der Plas A, Nijweide PJ. Function of osteocytes in bone: their role in mechanotransduction. J Nutr. July 1995;125(7)(suppl):S2020-S2023.
2006	Feng JQ, Ward LM, Liu S, Lu Y, Xie Y, Yuan B, Yu X, Rauch F, Davis SI, Zhang S, Rios H, Drezner MK, Quarles LD, Bonewald LF, White KE. Loss of DMP1 causes rickets and osteomalacia and identifies a role for osteocytes in mineral metabolism. Nat Genet. November 2006;38(11):1310-1315.
2021	Wilmoth RL. Development of a 3D Ex Vivo Culture System to Study Osteocyte Mechanobiology [PhD thesis]. University of Colorado; 2021.
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2007	Malone AMD. Mechanotransduction Mechanisms in Bone Cells [PhD thesis]. Stanford University; August 2007.
2010	Thompson WR. Perlecan Modulates the Function of the Osteocyte Lacuno-Canalicular System [PhD thesis]. University of Delaware; 2010.
2023	Halloran DR. Investigating Aberrant Bone Morphogenetic Protein-2 Signaling in Aged C57bl/6 Mice That is Reflective of Osteoporotic Patients [PhD thesis]. University of Delaware; Spring 2023.
2020	Kegelman CD. Combinatorial Roles of Skeletal Cell YAP and TAZ in Bone Growth, Remodeling, and Repair [PhD thesis]. Philadelphia, PA: University of Pennsylvania; 2020.
2015	Ng AH-M. A Mouse Model of Adynamic Bone Disease (ABD) and Its Consequences on Bone Quality With Age [PhD thesis]. University of Toronto; 2015.