Osteocyte differentiation in the tibia of newborn rabbit: an ultrastructural study of the formation of cytoplasmic processes

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Palumbo, Carla¹; Palazzini, Silvana¹; Zaffe, Davide¹; Marotti, Gastone¹

Osteocyte differentiation in the tibia of newborn rabbit: an ultrastructural study of the formation of cytoplasmic processes

Acta Anat. 1990;137(4):350-358

©1990 Karger AG, Basel

Affiliations

¹Istituto di Anatomia umana normale, Università di Modena, Italia
Abstract and keywords

The morphological changes undergone by the osteoblast at the ultrastructural level, during its differentiation into osteocyte, were studied in the primary parallel-fibred bone of the newborn rabbit by means of incomplete three-dimensional reconstruction from partially serial-sectioned preosteocytes. The findings obtained suggest that the formation of osteocyte cytoplasmic processes is an asynchronous and asymmetrical phenomenon that seems to precede the mineralization of the organic matrix and to give rise to an asymmetrical mature osteocyte. The functions of cytoplasmic processes as regards bone formation, cell nutrition and osteoblast modulation are discussed. The mechanism by which the osteoblast ‘enters’ the bone matrix is hypothesized.

Keywords: Osteocyte differentiation; Osteoblast; Preosteocyte; Cytoplasmic processes; Mineralizing surface; Newborn rabbit
Links

DOI: 10.1159/000146907

PubMed: 2368590

WoS: A1990DD40800010
History

Received: 1989-04-04

Accepted: 1989-07-03

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Cited By (30)

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1990	Palumbo C, Palazzini S, Marotti G. Morphological study of intercellular junctions during osteocyte differentiation. Bone. 1990;11(6):401-406.
1996	Mullender MG, van der Meer DD, Huiskes R, Lips P. Osteocyte density changes in aging and osteoporosis. Bone. February 1996;18(2):109-113.
2001	Kamioka H, Honjo T, Takano-Yamamoto T. A three-dimensional distribution of osteocyte processes revealed by the combination of confocal laser scanning microscopy and differential interference contrast microscopy. Bone. February 2001;28(2):145-149.
2002	Qiu S, Rao DS, Palnitkar S, Parfitt AM. Age and distance from the surface but not menopause reduce osteocyte density in human cancellous bone. Bone. August 2002;31(2):313-318.
2005	Sugawara Y, Kamioka H, Honjo T, Tezuka K-i, Takano-Yamamoto T. Three-dimensional reconstruction of chick calvarial osteocytes and their cell processes using confocal microscopy. Bone. May 2005;36(5):877-883.
2010	Schneider P, Meier M, Wepf R, Müller R. Towards quantitative 3d imaging of the osteocyte lacuno-canalicular network. Bone. November 2010;47(5):848-858.
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1990	Marotti G, Cane V, Palazzini S, Palumbo C. Structure-function relationships in the osteocyte. Ital J Min Electrol Metabol. 1990;4(2):93-106.
1997	Kato Y, Windle JJ, Koop BA, Mundy GR, Bonewald LF. Establishment of an osteocyte‐like cell line, MLO‐Y4. J Bone Miner Res. December 1997;12(12):2014-2023.
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2003	Gluhak-Heinrich J, Ye L, Bonewald LF, Feng JQ, MacDougall M, Harris SE, Pavlin D. Mechanical loading stimulates dentin matrix protein 1 (DMP1) expression in osteocytes in vivo. J Bone Miner Res. May 2003;18(5):807-817.
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1995	Burger EH, Klein-Nulend J, Van Der Plas A, Nijweide PJ. Function of osteocytes in bone: their role in mechanotransduction. J Nutr. July 1995;125(7)(suppl):S2020-S2023.
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2010	Thompson WR. Perlecan Modulates the Function of the Osteocyte Lacuno-Canalicular System [PhD thesis]. University of Delaware; 2010.
2023	Halloran DR. Investigating Aberrant Bone Morphogenetic Protein-2 Signaling in Aged C57bl/6 Mice That is Reflective of Osteoporotic Patients [PhD thesis]. University of Delaware; Spring 2023.
2020	Kegelman CD. Combinatorial Roles of Skeletal Cell YAP and TAZ in Bone Growth, Remodeling, and Repair [PhD thesis]. Philadelphia, PA: University of Pennsylvania; 2020.
2015	Ng AH-M. A Mouse Model of Adynamic Bone Disease (ABD) and Its Consequences on Bone Quality With Age [PhD thesis]. University of Toronto; 2015.