Effects of fluid shear stress on bone cells

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Williams, J. L.¹; Iannotti, J. P.¹; Ham, A.¹; Bleuit, J.¹; Chen, J. H.¹

Effects of fluid shear stress on bone cells

Biorheology. March–April 1994;31(2):163-170

©1994 Elsevier Science Ltd

Affiliations

¹Department of Orthopaedic Surgery, University of Pennsylvania, Philadelphia, PA 19104, USA
Abstract and keywords

Neonatal rat calvarial cells (osteoblast-enriched) were cultured in monolayer on glass slides and subjected to a step increase in shear stress ranging from 3 to 60 dyne/cm² in a laminar flow chamber. The level of intracellular free calcium ion concentration was monitored using Fura-2 fluorescence during the application of stress. Application of a step increase in stress resulted in a transient increase in calcium starting about 10–20 s after turning on the pump, reaching a peak in about 35 s and declining thereafter, despite the continued shear stress, and reaching near baseline values in about 100 s. No change in cytoplasmic calcium was observed with a step increase in shear stress of 3 dyne/cm². Between 6 dyne/cm² and 60 dyne/cm², the magnitude of the calcium response increased with the applied shear stress.

Keywords: Flow; signal transduction; mechanoreceptor; mechanical stimulation
Links

DOI: 10.3233/bir-1994-31204

PubMed: 8729478

WoS: A1994NF81800003
History

Received: 1993-06-10

Accepted: 1994-01-13

Cited Works (7)

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Cited By (23)

Year	Entry
1994	Zeng Y, Cowin SC, Weinbaum S. A fiber matrix model for fluid flow and streaming potentials in the canaliculi of an osteon. Ann Biomed Eng. May–June 1994;22(3):280-292.
2009	Fritton SP, Weinbaum S. Fluid and solute transport in bone: flow-induced mechanotransduction. Annu Rev Fluid Mech. 2009;41:347-374.
2004	Bacabac RG, Smit TH, Mullender MG, Dijcks SJ, Van Loon JJWA, Klein-Nulend J. Nitric oxide production by bone cells is fluid shear stress rate dependent. Biochem Biophys Res Commun. March 19, 2004;315(4):823-829.
2008	Bonewald LF, Johnson ML. Osteocytes, mechanosensing and Wnt signaling. Bone. April 2008;42(4):606-615.
1995	Hung T Clark, Pollack R Solomon, Reilly TM, Brighton T Carl. Real-time calcium response of cultured bone cells to fluid flow. Clin Orthop Relat Res. April 1995;313:256-269.
2012	Thompson WR, Rubin CT, Rubin J. Mechanical regulation of signaling pathways in bone. Gene. July 25, 2012;503(2):179-193.
1995	Cowin SC, Weinbaum S, Zeng Y. A case for bone canaliculi as the anatomical site of strain generated potentials. J Biomech. November 1995;28(11):1281-1297.
1996	Hung CT, Allen FD, Pollack SR, Brighton CT. What is the role of the convective current density in the real-time calcium response of cultured bone cells to fluid flow? J Biomech. November 1996;29(11):1403-1409.
1996	Hung CT, Allen FD, Pollack SR, Brighton CT. Intracellular Ca²⁺ stores and extracellular Ca²⁺ are required in the real-time Ca²⁺ response of bone cells experiencing fluid flow. J Biomech. November 1996;29(11):1411-1417.
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2000	Fritton SP, McLeod KJ, Rubin CT. Quantifying the strain history of bone: spatial uniformity and self-similarity of low-magnitude strains. J Biomech. March 2000;33(3):317-325.
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