Effects of fluid shear stress on bone cells

wbldb

Williams, J. L.¹; Iannotti, J. P.¹; Ham, A.¹; Bleuit, J.¹; Chen, J. H.¹

Effects of fluid shear stress on bone cells

Biorheology. March–April 1994;31(2):163-170

©1994 Elsevier Science Ltd

Affiliations

¹Department of Orthopaedic Surgery, University of Pennsylvania, Philadelphia, PA 19104, USA
Abstract and keywords

Neonatal rat calvarial cells (osteoblast-enriched) were cultured in monolayer on glass slides and subjected to a step increase in shear stress ranging from 3 to 60 dyne/cm² in a laminar flow chamber. The level of intracellular free calcium ion concentration was monitored using Fura-2 fluorescence during the application of stress. Application of a step increase in stress resulted in a transient increase in calcium starting about 10–20 s after turning on the pump, reaching a peak in about 35 s and declining thereafter, despite the continued shear stress, and reaching near baseline values in about 100 s. No change in cytoplasmic calcium was observed with a step increase in shear stress of 3 dyne/cm². Between 6 dyne/cm² and 60 dyne/cm², the magnitude of the calcium response increased with the applied shear stress.

Keywords: Flow; signal transduction; mechanoreceptor; mechanical stimulation
Links

DOI: 10.3233/bir-1994-31204

PubMed: 8729478

WoS: A1994NF81800003
History

Received: 1993-06-10

Accepted: 1994-01-13

Cited Works (7)

Year	Entry
1994	Weinbaum S, Cowin SC, Zeng Y. A model for the excitation of osteocytes by mechanical loading-induced bone fluid shear stresses. J Biomech. March 1994;27(3):339-360.
1990	Murray DW, Rushton N. The effect of strain on bone cell prostaglandin E₂ release: a new experimental method. Calcif Tiss Int. July 1990;47(1):35-39.
1991	Jones DB, Nolte H, Scholübbers J-G, Turner E, Veltel D. Biochemical signal transduction of mechanical strain in osteoblast-like cells. Biomaterials. March 1991;12(2):101-110.
1984	Binderman I, Shimshoni Z, Somjen D. Biochemical pathways involved in the translation of physical stimulus into biological message. Calcif Tiss Int. March 1984;36(1):S82-S85.
1991	Reich KM, Frangos JA. Effect of flow on prostaglandin E₂ and inositol trisphosphate levels in osteoblasts. Am J Physiol. September 1991;261(3):C428-C432.
1990	Reich KM, Gay CV, Frangos JA. Fluid shear stress as a mediator of osteoblast cyclic adenosine monophosphate production. J Cell Physiol. April 1990;143(1):100-103.
1984	Yeh C-K, Rodan GA. Tensile forces enhance prostaglandin E synthesis in osteoblastic cells grown on collagen ribbons. Calcif Tiss Int. March 1984;36(1):S67-S71.

Cited By (25)

Year	Entry
2001	Knothe Tate ML. Bone poroelasticity. In: Cowin SC, ed. Bone Mechanics Handbook. 2nd ed. Boca Raton, FL: CRC Press; 2001:22-1–22-29.
2001	Cowin SC, Moss ML. Mechanosensory mechanisms in bone. In: Cowin SC, ed. Bone Mechanics Handbook. 2nd ed. Boca Raton, FL: CRC Press; 2001:29-1–29-17.
1994	Zeng Y, Cowin SC, Weinbaum S. A fiber matrix model for fluid flow and streaming potentials in the canaliculi of an osteon. Ann Biomed Eng. May–June 1994;22(3):280-292.
2009	Fritton SP, Weinbaum S. Fluid and solute transport in bone: flow-induced mechanotransduction. Annu Rev Fluid Mech. 2009;41:347-374.
2004	Bacabac RG, Smit TH, Mullender MG, Dijcks SJ, Van Loon JJWA, Klein-Nulend J. Nitric oxide production by bone cells is fluid shear stress rate dependent. Biochem Biophys Res Commun. March 19, 2004;315(4):823-829.
2008	Bonewald LF, Johnson ML. Osteocytes, mechanosensing and Wnt signaling. Bone. April 2008;42(4):606-615.
1995	Hung T Clark, Pollack R Solomon, Reilly TM, Brighton T Carl. Real-time calcium response of cultured bone cells to fluid flow. Clin Orthop Relat Res. April 1995;313:256-269.
2012	Thompson WR, Rubin CT, Rubin J. Mechanical regulation of signaling pathways in bone. Gene. July 25, 2012;503(2):179-193.
1995	Cowin SC, Weinbaum S, Zeng Y. A case for bone canaliculi as the anatomical site of strain generated potentials. J Biomech. November 1995;28(11):1281-1297.
1996	Hung CT, Allen FD, Pollack SR, Brighton CT. What is the role of the convective current density in the real-time calcium response of cultured bone cells to fluid flow? J Biomech. November 1996;29(11):1403-1409.
1996	Hung CT, Allen FD, Pollack SR, Brighton CT. Intracellular Ca²⁺ stores and extracellular Ca²⁺ are required in the real-time Ca²⁺ response of bone cells experiencing fluid flow. J Biomech. November 1996;29(11):1411-1417.
1999	Wang L, Fritton SP, Cowin SC, Weinbaum S. Fluid pressure relaxation depends upon osteonal microstructure: modeling an oscillatory bending experiment. J Biomech. July 1999;32(7):663-672.
2000	Fritton SP, McLeod KJ, Rubin CT. Quantifying the strain history of bone: spatial uniformity and self-similarity of low-magnitude strains. J Biomech. March 2000;33(3):317-325.
2001	You L, Cowin SC, Schaffler MB, Weinbaum S. A model for strain amplification in the actin cytoskeleton of osteocytes due to fluid drag on pericellular matrix. J Biomech. November 2001;34(11):1375-1386.
1999	McAllister TN, Frangos JA. Steady and transient fluid shear stress stimulate NO release in osteoblasts through distinct biochemical pathways. J Bone Miner Res. June 1999;14(6):930-936.
1997	Zhang D. Electromechanical Mechanisms of Bone Remodeling [PhD thesis]. New York, NY: The City University of New York; 1997.
2002	Wang L. Investigating the Role of Interstitial Fluid Flow in Bone Adaptation and Metabolism [PhD thesis]. New York, NY: The City University of New York; 2002.
2002	You L. A New View of Mechanotransduction in Bone Cells [PhD thesis]. New York, NY: The City University of New York; 2002.
2004	Mi LY. Analysis of Avian Bone Response to Mechanical Loading Using a Computational Connected Network [PhD thesis]. New York, NY: The City College of New York; 2004.
2005	Yoon YJ. Estimates of Bone Elastic Constants At Sequential Hierarchical Levels [PhD thesis]. New York, NY: The City University of New York; 2005.
2006	Han Y. On the Transmission of Fluid Forces to the Actin Cytoskeleton of Bone and Endothelial Cells [PhD thesis]. New York, NY: The City University of New York; 2006.
2000	Ryder KD. Parathyroid Hormone Modulates the Response of Osteoblast-Like Cells to Mechanical Stimulation [PhD thesis]. Indianapolis, IN: Indiana University; May 2000.
2006	Hannay G. Mechanical and Electrical Environments to Stimulate Bone Cell Development [PhD thesis]. Queensland University of Technology; August 2006.
2004	LaMothe JM. Functional Adaptation of Bone [PhD thesis]. Calgary, AB: University of Calgary; September 2004.
1996	Beck BR. The Relationship of Streaming Potential Magnitude to Strain and Periosteal Modeling [PhD thesis]. Eugene, OR: University of Oregon; December 1996.