Nanoindentation and storage of teeth

Habelitz, Stefan<sup>1</sup>; Marshall, Jr, Grayson W.<sup>1</sup>; Balooch, Mehdi<sup>2</sup>; Marshall, Sally J.<sup>1</sup>

Affiliations

¹Department of Preventive and Restorative Dental Sciences, University of California, 707 Parnassus Avenue D-2250, San Francisco, CA 94143 0758, USA

²Department of Chemistry and Materials Science, Lawrence Livermore National Laboratory, Livermore, CA 94550, USA

Abstract and keywords

This study determined changes in nanomechanical properties of dentin and enamel during storage in deionized water, calcium chloride buffered saline solution and Hank's balanced salts solution (HBSS). Atomic force microscopy based nanoindentation showed that storing teeth in deionized water or CaCl₂-solution resulted in a large decrease in elastic modulus and hardness. At 1 day a decrease in the mechanical properties values of up to 20% and 30% was observed for enamel and dentin, respectively. After 1 week, mechanical properties dropped below 50% of their starting values, which is attributed to a demineralization process during storage. In contrast, storing teeth in HBSS did not significantly alter the mechanical properties for a time interval of 2 weeks. The use of HBSS for storage of samples from teeth is recommended.

Keywords: Teeth; Nanoindentation; Atomic force microscopy; Mineral dissolution; Storage

Links

DOI: 10.1016/S0021-9290(02)00039-8

PubMed: 12052404

WoS: 000176608900016

History

Accepted: 2002-02-23

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Cited By (16)

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