In vivo axial loading of the mouse tibia is a common model of bone loading used to study bone cell response, mechanoadaptation and the effects of diseases such as osteoporosis and other genetic factors on bone mechanobiology. the changes that can be seen during in vivo loading of the tibia are often compared to the maximum strain value on the surface of the bone during loading. Strain alone does not provide an accurate prediction of bone response, which is more likely related to time varying parameters such as fluid shear. The fluid flow within the bone can be modeled using poroelastic models allowing researchers to predict the mechanical stimuli that will affect bone cells in in vivo experiments and tune their loading profiles in order to ensure that equivalent stimuli are applied to cells in all populations under study. Whole-bone poroelastic models were used to compare the effects of age-related changes in bone geometry on fluid flow under a variety of loading profiles with varying loading rates, periods, and profiles. Old tibiae require higher loading to generate equivalent surface strains, yet the maximum fluid velocity generated by all loading profiles is greater in young bones than in old. Altering the period or loading rate of the load profiles used between populations can lead to equal mechanical stimuli on the bone cells and a better prediction of their response to applied loads.