Cancellous bone is a highly porous, heterogeneous, and anisotropic material which can be found at the epiphyses of long bones and in the vertebral bodies. The hierarchical architecture makes cancellous bone a prime example of a lightweight natural material that combines strength with toughness. Better understanding the mechanics of cancellous bone is of interest for the diagnosis of bone diseases, the evaluation of the risk of fracture, and for the design of artificial bones and bone scaffolds for tissue engineering. A multiscale optimization method to maximize the stiffness of artificial bones using biomimetic cellular microstructures described by a finite set of geometrical micro-parameters is presented here. The most outstanding characteristics of its implementation are the use of:​ an interior point optimization algorithm, a precalculated response surface methodology for the evaluation of the elastic tensor of the microstructure as an analytical function of the micro-parameters, and the adjoint method for the computation of the sensitivity of the macroscopic mechanical response to the variation of the micro-parameters. The performance and effectiveness of the tool are evaluated by solving a problem that consists in finding the optimal distribution of the microstructures for a proximal end of a femur subjected to physiological loads. Two strategies for the specification of the solid volume fraction constraints are assessed. The results are compared with data of a computed tomography study of an actual human bone. The model successfully predicts the main features of the spatial arrangement of the trabecular and cortical microstructures of the natural bone.