Osteoporosis-related variations of trabecular bone properties of proximal human humeral heads at different scale lengths

Molino, Giulia; Dalpozzi, Alessio; Ciapetti, Gabriela; Lorusso, Massimo; Novara, Chiara; Cavallo, Marco; Baldini, Nicola; Giorgis, Fabrizio; Fiorilli, Sonia; Vitale-Brovarone, Chiara

Affiliations

¹Politecnico di Torino, Department of Applied Science and Technology, Corso Duca degli Abruzzi 24, 10129, Torino, Italy

²Laboratory for Orthopaedic Pathophysiology and Regenerative Medicine, IRCCS Istituto Ortopedico Rizzoli, Bologna, Italy

³Center for Sustainable Future Technologies@Polito, Istituto Italiano di Tecnologia, Via Livorno 60, 10145, Torino, Italy

⁴Shoulder and Elbow Surgery Unit, IRCCS Istituto Ortopedico Rizzoli, Bologna, Italy

⁵Laboratory for Orthopaedic Pathophysiology and Regenerative Medicine, IRCCS Istituto Ortopedico Rizzoli, & Department of Biomedical and Neuromotor Sciences, University of Bologna, Italy

Abstract and keywords

Osteoporosis (OP) is a skeletal disorder responsible for the weakening of the bone structure and, consequently, for an increased fracture risk in the elderly population. In the past, bone mineral density (BMD) variation was considered the best OP indicator, but recently the focus has shifted toward the variation of microstructural bone parameters. This work is based on the characterisation of 8-mm cylindrical biopsies harvested from proximal humeral heads belonging to healthy and osteoporotic patients, in order to assess the OP-related variations of bone properties at different scale lengths. In particular, bone biopsies underwent micro-computed tomography analysis to study the most relevant features of bone architecture and extrapolate the tissue mineral density (TMD) value of bone trabeculae. Compression tests and nanoindentations were performed to investigate the macro- and micromechanical properties of bone biopsies, respectively. In addition, XRD analyses were performed to obtain the mean hydroxyapatite (HA) crystallite size, while Raman spectroscopy investigated the collagen secondary structure. Thermogravimetric analysis was performed to evaluate the ratio between organic and inorganic phases.

From the obtained results, OP samples showed a more anisotropic and less interconnected structure responsible for reduced compression strength. From this, it can be supposed that OP caused an alteration of bone structure that led to inferior macroscopic mechanical properties. Furthermore, OP samples possessed higher TMD and bigger HA crystals that are correlated to an increase of the hardness value obtained by means of nanoindentation. This less controlled HA crystal growth is probably due to an alteration of the organic matrix structure, as revealed by the increase of the random coil contribution in the Raman spectra of the OP bone. This higher crystal content led to an increase in trabecular density and hardness. In conclusion, the obtained data showed that OP affects bone properties at different scale lengths causing an alteration of its morphological, structural and mechanical features.

Keywords: Osteoporosis; Humeral head; Micro-CT; Compression test; Nanoindentation; Raman spectroscopy

Links

DOI: 10.1016/j.jmbbm.2019.103373

PubMed: 31369957

WoS: 000496832200003

History

Received: 2019-01-29

Revised: 2019-04-01

Accepted: 2019-07-25

Online: 2019-07-26

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Cited By (3)

Year	Entry
2021	Ramírez A CE, Hurtado-Macías A, Talamantes R, Flores E, Ladrón de Guevara HP, Delgado JI, Estrella RA, Riestra JM, Montes JM, Esmonde-White K, Vardaki M, González-Hernández J, Viveros JM. Assessing mechanical behavior of ostrich and equine trabecular and cortical bone based on depth sensing indentation measurements. J Mech Behav Biomed Mater. May 2021;117:104404.
2020	O’Sullivan LM. Time-Sequence of Biomechanical Adaption in Trabecular Tissue During Estrogen Deficiency [PhD thesis]. National University of Ireland Galway; March 2020.
2021	Kusins J. A Multi-Level Mechanical Assessment of the Shoulder Coupled With Evaluation of Upper Extremity Predictive Finite Element Models [PhD thesis]. University of Western Ontario; 2021.