The anatomic complexity and heterogeneity of the human abdomen make it difficult to develop a biofidelic abdominal component in an anthropomorphic test device that can predict abdominal injuries in crash testing. Several previous studies have attempted to relate external physical parameters such as energy, external pressure, impact force, velocity, and compression to abdominal injury. While many of these approaches have been useful in developing response corridors for the abdomen as a whole, the interaction between different abdominal organs and the responses of individual organs are not well understood. This paper presents a method for measuring intravascular pressure gradients within isolated, physiologically pressurized human livers in response to blunt impact. Intravascular pressure data is presented from testing of a rigidly constrained, unembalmed liver specimen. Future work will include analysis of intra-liver pressure changes and injury outcomes in impact tests of intact post-mortem human subjects (PMHS). By matching the pressure gradients in the isolated liver testing to those observed in the intact PMHS tests, the boundary conditions describing the liver’s environment within the abdominal cavity could potentially be modeled.