Dynamic equibiaxial tension tests were conducted on cruciate tissue samples obtained from the colon of four human cadavers. Tissue samples were stretched to failure using a custom equibiaxial test device. Load, acceleration, sample thickness, and the displacement of optical markers on the sample surface were recorded throughout the duration of each test. The stress‐strain response was quantified for 21 colon tests conducted at an average maximum principal strain rate of 69.2 ± 16.0 s^-1. The data indicated regional and directional variations in colon material response. The average Green‐Lagrange failure strain was 0.158 ± 0.036 and 0.139 ± 0.039 in the circumferential and longitudinal directions, respectively. The average 2nd Piola Kirchhoff stress at failure was 2.35 ± 1.37 MPa in the circumferential direction and 3.20 ± 1.51 MPa in the longitudinal direction. An increased resistance to stretch was observed in the longitudinal direction with a greater average failure stress and lower average failure strain compared to the circumferential direction. Regional response variations included a lower tensile strength for the descending colon compared to the other regions in both the longitudinal and circumferential directions. Data from this study characterize the biomechanical response of the human post‐mortem colon at strain rates expected to be experienced in motor vehicle collisions.