3D histomorphometric quantification of trabecular bones by computed microtomography using synchrotron radiation

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Nogueira, L. P.; Braz, D.; Barroso, R. C.; Oliveira, L. F.; Pinheiro, C. J. G.; Dreossi, D.; Tromba, G.

3D histomorphometric quantification of trabecular bones by computed microtomography using synchrotron radiation

Micron. 2010;41(8):990-996

Abstract and keywords

Conventional bone histomorphometry is an important method for quantitative evaluation of bone microstructure. X-ray computed microtomography is a non-invasive technique, which can be used to evaluate histomorphometric indices in trabecular bones (BV/TV, BS/BV, Tb.N, Tb.Th, Tb.Sp). In this technique, 3D images are used to quantify the whole sample, differently from the conventional one, in which the quantification is performed in 2D slices and extrapolated for 3D case. In this work, histomorphometric quantification using synchrotron 3D X-ray computed microtomography was performed to quantify the bone structure at different skeletal sites as well as to investigate the effects of bone diseases on quantitative understanding of bone architecture. The images were obtained at Synchrotron Radiation for MEdical Physics (SYRMEP) beamline, at ELETTRA synchrotron radiation facility, Italy. Concerning the obtained results for normal and pathological bones from same skeletal sites and individuals, from our results, a certain declining bone volume fraction was achieved. The results obtained could be used in forming the basis for comparison of the bone microarchitecture and can be a valuable tool for predicting bone fragility.

Keywords: Histomorphometry; Synchrotron radiation computed microtomography; Bone microstructure; Image processing
Links

DOI: 10.1016/j.micron.2010.06.013

PubMed: 20673730

WoS: 000282673500010

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Cited By (2)

Year	Entry
2020	Acciaioli A, Falco L, Balean M. Measurement of apparent mechanical properties of trabecular bone tissue: accuracy and limitation of digital image correlation technique. J Mech Behav Biomed Mater. March 2020;103:103542.
2013	Abdullah Chisti KBM. Texture Analysis of Diffraction Enhanced Synchrotron Images of Trabecular Bone At the Wrist [Master's thesis]. University of Saskatchewan; August 2013.