This study aimed to investigate anatomical variations within the medial collateral ligament complex of the human ankle joint. Osteoligamentous preparations of 40 ankles from 20 cadavers were studied. Six different component bands of the medial collateral ligament complex were observed:​ four superficial bands (tibiospring, tibionavicular, superficial posterior tibiotalar, and tibiocalcaneal ligaments), of which only the tibiospring and tibionavicular ligaments were constant, and two deep bands (deep posterior tibiotalar and deep anterior tibiotalar ligaments), of which only the deep posterior tibiotalar ligament was constant. No pattern was observed in the distribution of additional bands. A consistent and universally accepted system for naming the various bands of the medial collateral ligament is necessary to enable easy and accurate comparisons between studies. We suggest that this system should be based on the attachments of the ligaments.