Effects of risedronate on trabecular microstructure and biomechanical properties in ovariectomized rat tibia

Ito, Masako; Nishida, Akifumi; Aoyagi, Kiyoshi; Uetani, Masataka; Hayashi, Kuniaki; Kawase, Masahiro

Affiliations

¹Department of Radiology, Nagasaki University School of Medicine, 1-7-1 Sakamoto, 852-8501 Nagasaki, Japan

²Department of Public Health, Nagasaki University School of Medicine, Nagasaki, Japan

³Drug Analysis & Pharmacokinetics Research Laboratories, Pharmaceutical Research Division, Takeda Chemical Industries Ltd., Osaka, Japan

Abstract and keywords

We determined the effect of risedronate on the trabecular microstructure of ovariectomized rat tibiae, using micro-computed tomography, in order to investigate how changes in microstructure contribute to biomechanical properties. Fifty 18-week-old rats underwent sham operation (n=10) or ovariectomy (OVX) (n=40). The OVX rats were further divided into four groups (n=10 for each group) and treated with risedronate at doses of 0, 0.1, 0.5 or 2.5 mg/kg for 9 months. OVX caused deterioration of three-dimensional trabecular microstructure, notably structure model index (SMI) and connectivity density, while treatment of OVX rats with risedronate at 0.5 and 2.5 mg/kg improved those deleterious microstructural changes. Biomechanical property, as assessed by finite element analysis (FEA), correlated significantly with trabecular bone volume fraction (BV/TV), and the correlation further increased substantially when microstructural parameters were added, especially SMI and connectivity density, with risedronate therapy. Thus, it is suggested that, in addition to increasing bone mass, risedronate improves biomechanical property by maintaining a plate-like structure as well as connectivity of trabeculae.

Keywords: Biomechanical property; Connectivity; Finite element analysis (FEA); Microstructure; Risedronate

Links

DOI: 10.1007/s00198-004-1802-3

PubMed: 15711780

WoS: 000231604500005

History

Received: 2003-08-19

Accepted: 2004-10-21

Online: 2005-02-12

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2020	Harris D, Garrett K, Uppuganti S, Creecy A, Nyman JS. The BALB/c mouse as a preclinical model of the age-related deterioration in the lumbar vertebra. Bone. August 2020;137:115438.
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2011	Nazarian A, Arroyo FJA, Rosso C, Aran S, Snyder BD. Tensile properties of rat femoral bone as functions of bone volume fraction, apparent density and volumetric bone mineral density. J Biomech. September 2, 2011;44(13):2482-2488.
2019	Solitro GF, Mainnemare F, Amirouche F, Mehta A. A novel technique with reduced computed tomography exposure to predict vertebral compression fracture: a finite element study based on rat vertebrae. Med Biol Eng Comput. April 2019;57(4):795-805.
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