Cancer study and drug selection research attract more and more researchers, which need a significant laboratory technique, named cell injection. Hundreds of cells are loaded on devices and injected to investigate the behavior of the cells. Traditionally, cell injection is performed manually, which leads to human fatigue, is time-consuming and has a low success rate. Therefore, a system which can replicate the actions of what technicians do, such as to aspirate cells, transfer cells, immobilize cells, and release cells automatically, is needed. This system must be accurate, reliable, and efficient and operate without human intervention. A cell-transfer-cover and a cell-holder have been fabricated and a cell injection system has been set up to investigate the performance of the newly created device. Simulations and experiments have proven that this system would carry out the entire process of cell injection with the result of enhancing the speed of this important activity.