Quantification of sulfated glycosaminoglycans in chondrocyte/alginate cultures, by use of 1,9-dimethylmethylene blue

Enobakhare, Brian O.; Bader, Dan L.; Lee, David A.

Affiliations

¹IRC in Biomedical Materials, Institute of Orthopaedics, UCL, Brockley Hill, Stanmore, Middlesex HA7 4LP, United Kingdom

²IRC in Biomedical Materials, Queen Mary and Westfield College, University of London, Mile End Road, London E1 4NS, United Kingdom

Links

DOI: 10.1006/abio.1996.0502

PubMed: 8954546

WoS: A1996VX62200028

History

Received: 1996-07-1

Cited Works (2)

Year	Entry
1986	Farndale RW, Buttle DJ, Barrett AJ. Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue. Biochim Biophys Acta. September 4, 1986;883(2):173-177.
1982	Farndale RW, Sayers CA, Barrett AJ. A direct spectrophotometric microassay for sulfated glycosaminoglycans in cartilage cultures. Connect Tissue Res. 1982;9(4):247-248.

Cited By (38)

Year	Entry
2004	Awad HA, Wickham MQ, Leddy HA, Gimble JM, Guilak F. Chondrogenic differentiation of adipose-derived adult stem cells in agarose, alginate, and gelatin scaffolds. Biomaterials. July 2004;25(16):3211-3222.
2000	Mauck RL, Soltz MA, Wang CCB, Wong DD, Chao P-HG, Valhmu WB, Hung CT, Ateshian GA. Functional tissue engineering of articular cartilage through dynamic loading of chondrocyte-seeded agarose gels. J Biomech Eng. June 2000;122(3):252-260.
2017	Marble HD. Gene Expression-Based Identification and Isolation of Cellular Subpopulations [PhD thesis]. Brown University; May 2017.
2003	Mauck RL. Functional Tissue Engineering of Articular Cartilage: The Effect of Physical Forces on the in Vitro Growth of Engineered Constructs [PhD thesis]. Columbia University; 2003.
2007	Wan Q. Fundamental Theoretical and Experimental Studies for Applications Toward Functional Tissue Engineering of Articular Cartilage [PhD thesis]. Columbia University; 2007.
2012	Khanarian NT. Scaffold Design and Optimization for Osteochondral Interface Tissue Engineering [PhD thesis]. Columbia University; 2012.
2014	Subramony SD. Nanofiber-Based Scaffold for Integrative Anterior Cruciate Ligament Reconstruction [PhD thesis]. Columbia University; 2014.
2016	Boushell MK. Scaffold Design and Optimization for Integrative Cartilage Repair [PhD thesis]. Columbia University; 2016.
2016	Zhang X. Nanofiber-Based Scaffold for Integrative Rotator Cuff Repair [PhD thesis]. Columbia University; 2016.
2019	Qu D. Tissue Engineering Strategies for Fibrocartilage Interface Regeneration [PhD thesis]. Columbia University; 2019.
2020	Mosher CZ. Interface Scaffold Design Principles for Integrative Cartilage Regeneration [PhD thesis]. Columbia University; 2020.
2010	Babalola OM. Effects of Physical Stimuli on Extracellular Matrix Assembly by Chondrocytes and Mesenchymal Stem Cells: Experimental and Finite Element Analyses [PhD thesis]. Ithaca, NY: Cornell University; May 2010.
2010	Ballyns JJ. Dynamic Loading of Anatomically Shaped Meniscal Constructs Generated Via MRI and Micro-CT Imaging [PhD thesis]. Ithaca, NY: Cornell University; August 2010.
2010	Degala S. Fluid Flow Regulates Articular Chondrocyte Mechanotransduction Pathways in 3D Culture [PhD thesis]. Ithaca, NY: Cornell University; May 2010.
2008	Gleghorn JP. Boundary Mode Frictional Properties of Articular Cartilage: Functional Implications of Lubricin Localization [PhD thesis]. Ithaca, NY: Cornell University; January 2008.
2014	Puetzer JL. Development of Engineered Menisci With Native-Like Organization Using High Density Collagen Gels and Mechanical Stimulation [PhD thesis]. Ithaca, NY: Cornell University; August 2014.
2017	McCorry MC. Tissue Engineering the Meniscus for Clinical Translation: Stem Cells, Fibers, and Attachments [PhD thesis]. Ithaca, NY: Cornell University; August 2017.
2018	Cohen BP. Overcoming Clinical Challenges to Tissue Engineering the Human Auricle [PhD thesis]. Ithaca, NY: Cornell University; December 2018.
2018	DiDomenico CD. Evaluating Macromolecular Transport in Articular Cartilage to Better Understand and Predict Transport of Arthritis Therapeutics [PhD thesis]. Ithaca, NY: Cornell University; August 2018.
2018	Mojica Santiago JA. Tissue Engineering of Cervical Intervertebral Discs: An in Vitro and in Vivo Pre-Clinical Canine Model of Total Disc Replacement [PhD thesis]. Ithaca, NY: Cornell University; August 2018.
2019	Diamantides NC. Development of Collagen Bioinks for Cartilage Bioprinting [PhD thesis]. Ithaca, NY: Cornell University; December 2019.
2019	Middendorf JM. Establishing New Standards for Cartilage Repair: The Structure-Function Relationship of Human Tissue Engineered Cartilage [PhD thesis]. Ithaca, NY: Cornell University; June 2019.
2020	Sloan SR Jr. Tissue-Engineered Annulus Fibrosus Repair and Nucleus Pulposus Augmentation in Pre-Clinical Models of Intervertebral Disc Degeneration [PhD thesis]. Ithaca, NY: Cornell University; May 2020.
2021	Lintz M. Tissue Engineering Strategies for Total Disc Replacement: Structure and Mechanical Function of the Intervertebral Disc [PhD thesis]. Ithaca, NY: Cornell University; August 2021.
2012	Diekman BO. Stem Cell-Based Strategies to Study, Prevent, and Treat Cartilage Injury and Osteoarthritis [PhD thesis]. Duke University; 2012.
2015	Nimeskern LG. Functional Characterization of Auricular Cartilage in Tissue Engineering and Regenerative Medicine [PhD thesis]. Swiss Federal Institute of Technology Zürich; 2015.
2005	Case ND. Oscillatory Compressive Loading Effects on Mesenchymal Progenitor Cells Undergoing Chondrogenic Differentiation in Hydrogel Suspension [PhD thesis]. Atlanta, GA: Georgia Institute of Technology; May 2005.
2005	Mouw JK. Mechanoregulation of Chondrocytes and Chondroprogenitors: The Role of TGF-Β and SMAD Signaling [PhD thesis]. Atlanta, GA: Georgia Institute of Technology; December 2005.
2007	Coleman R. Development of a Small Animal Model to Study Tissue Engineering Strategies for Growth Plate Defects [PhD thesis]. Atlanta, GA: Georgia Institute of Technology; August 2007.
2011	Jeon JE. Development of Zonal Cartilage Constructs: Effects of Chondrocyte Subpopulation, Compressive Stimulation, and Culture Models [PhD thesis]. Queensland University of Technology; 2011.
2015	Kuaha K. Environments for Zonal Cartilage Tissue Engineering [PhD thesis]. Queensland University of Technology; 2015.
2013	Zheng Ch. Effects of Biochemical Factors and Mechanical Loading on Chondrogenesis of Human Adipose-Derived Stem Cells [PhD thesis]. Stanford University; December 2013.
2006	Chawla K. Mechanisms of Articular Cartilage Defect Repair in Vivo After Implantation of Stratified Cartilaginous Tissue [PhD thesis]. San Diego (UCSD), University of California; 2006.
2009	Hsieh-Bonassera ND. Cartilage Tissue Engineering With Human Chondrocytes from Osteoarthritic Knees and a Semi-Permeable Membrane [PhD thesis]. San Diego (UCSD), University of California; 2009.
2010	Williams GM. Biophysical and Biochemical Regulation of the Shape, Size, and Maturity of Calf Articular Cartilage [PhD thesis]. San Diego (UCSD), University of California; 2010.
2013	Laws CJ. Mechanisms of Disc Herniation in Astronauts Following Spaceflight [PhD thesis]. San Francisco, University of California; 2013.
2017	Ouyang A. Effects of Design Factors and Microenvironment on Mesenchymal Stem Cells and Nucleus Pulposus Cells for Intervertebral Disc Tissue Engineering [PhD thesis]. San Francisco, University of California; 2017.
2020	Horne D. Development of Novel Energy-Based Musculoskeletal Therapies [PhD thesis]. San Francisco, University of California; 2020.