Background/Objective:​ Osteoarthritis is a common chronic disease of the joints characterised by the degeneration of articular cartilages and subchondral bone. The most common diagnostic imaging used clinically is X-ray;​ however, it cannot directly image cartilage. Magnetic resonance imaging (MRI) is well suited for cartilage imaging, but it requires costly and lengthy scans. For preclinical work, microcomputed tomography provides high spatial resolution and contrast for bone, however, its standard application is not well suited for cartilage imaging.

Methods:​ We performed a preliminary investigation into the use of dual-energy X-ray microscopy (XRM) for cartilage imaging and analysis of a rabbit knee, and compared it to the MRI results from 9.4 T and 1.0 T small-animal scanners.

Results:​ The XRM images offer a higher image resolution (∼25 μm nominal isotropic resolution) compared with the MRI (50–86 μm in plane, and 250 μm slice thickness). The cartilage-thickness measurements using the dual-energy XRM are on average 3.8% (femur) and 5.1% (tibia) thicker estimates than the 9.4 T MRI results. The cartilage-thickness measurements using the 1.0 T MRI are on average 10.9% (femur) and 2.3% (tibia) thinner estimates than the 9.4 T MRI results.

Conclusion:​ Our results suggest that the dual-energy XRM for articular-cartilage analysis is feasible and comparable to the MRI. This technology will provide good support for high-resolution animal-osteoarthritis studies, and in the future, it may be possible to apply dual energy in a clinical setting.