Past LE landing studies have investigated the effect of several biomechanical factors on lower extremity loads in an effort to characterize their roles in injury potential. Neuromuscular fatigue is also thought to increase injury potential, but its effect on lower extremity loads has received limited attention in the literature. The purpose of this investigation was to evaluate the effects of fatigue on ground reaction force characteristics, and lower extremity kinematics, kinetics, and energetics during landing. A muscle temperature compensation technique was first established to allow EMG fatigue measurements during a high-intensity activity. This technique was used to monitor subject fatigue during the landing part of the study. Twelve male subjects who were physically active and had no recent history of lower extremity injury volunteered to participate. Subjects were asked to repeatedly perform a sequence of single-leg landings (25 cm height) and single-leg squats until exhaustion of the knee extensor muscles. EMG, kinetic, and kinematic data were collected during the activity. Angular impulse and energy absorption at the hip, knee, and ankle were calculated for the landing movements using an inverse dynamics analysis technique. EMG fatigue analysis demonstrated that subjects experienced fatigue of the quadriceps muscle. The ground reaction force peak and impulse decreased, and sagittal plane joint flexion increased at the hip, knee, and ankle with fatigue. These findings suggest a decreased ability to decelerate upon landing when fatigued. Sagittal plane extensor impulse and energy absorption increased at the hip, and decreased at the knee, and ankle with fatigue. Data also suggested a change in landing strategy, based on a reversal in load redistribution patterns, as fatigue progressed. The redistribution of extensor impulse and energy absorption from distal to proximal with fatigue may allow larger proximal muscles to assist the smaller distal muscle in extensor torque production and energy absorption. This may also have the negative effect of increasing the mechanical energy exposure at the knee and ankle, thereby increasing the injury potential at these joints.