Correlation of mineral density and elastic modulus of dog dentin using μ-CT and nanoindentation

Soukup, Jason W.; Jeffery, Justin; Drizin, Sienna R.; Hetzel, Scott J.; Stone, Donald S.; Eriten, Melih; Ploeg, Heidi-Lynn; Henak, Corinne R.

Affiliations

¹Department of Surgical Sciences, University of Wisconsin-Madison, School of Veterinary Medicine, Madison, WI, USA

²Carbone Cancer Center, University of Wisconsin-Madison, School of Medicine and Public Health, Madison, WI, USA

³Department of Biostatistics and Medical Informatics, University of Wisconsin-Madison, School of Medicine and Public Health, Madison, WI, USA

⁴Department of Materials Science and Engineering, University of Wisconsin-Madison, College of Engineering, Madison, WI, USA

⁵Department of Mechanical Engineering, University of Wisconsin-Madison, College of Engineering, Madison, WI, USA

⁶Department of Mechanics and Materials Engineering, Queen’s University, Kingston, ON, Canada

⁷Department of Orthopedics and Rehabilitation, University of Wisconsin-Madison, School of Medicine and Public Health, Madison, WI, USA

Abstract and keywords

This study sought to 1) investigate the spatial distribution of mineral density of dog dentin using µ-CT and 2) characterize the relationship between the elastic modulus and mineral density of dog dentin using nanoindentation and µ-CT. Maxillary canine teeth of 10 mature dogs were scanned with a µ-CT then sectioned in the transverse and vertical planes and tested using nanoindentation. Spatial distribution of mineral density and elastic modulus was quantified. Results demonstrated significant spatial variation in mineral density and elastic modulus. Mineral density and elastic modulus generally increased from the dentin-pulp interface to the dentino-enamel junction and from the crown base to the crown tip. Significant site dependent correlations between mineral density and elastic modulus were determined (0.021 > R² > 0.408). The results of this study suggest that while mineral density is a mediator of elastic modulus, other mediators such as collagen content may contribute to the mechanical behavior of dog dentin.

Keywords: Dentin; Microcomputed tomography; µ-CT; Nanoindentation; Elastic modulus; Elasticity-density relationship; Mineral density; ρ_QCT

Links

DOI: 10.1016/j.jbiomech.2023.111434

History

Accepted: 2023-01-3

Online: 2023-01-7

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