Ligamentous injuries range in severity from a simple sprain to a complete rupture. Although sprains occur more frequently than complete failures, only a few studies have investigated the phenomena of these subfailure injuries. The purpose of our study was to document the changes in the load-deformation curve until the failure point, after the ligament has been subjected to an 80% subfailure stretch. Thirteen paired fresh rabbit bone-anterior cruciate ligament-bone preparations were used. One of the pairs (control) was stretched until failure;​ the other (experimental) was first stretched to 80% of the failure deformation of the control and then stretched to failure. Comparisons were made between the load-deformation curves of the experimental and control specimens. The nonlinear load-deformation curves were characterized by eight parameters:​ failure load (F_fail), failure deformation (D_fail), energy until failure (E_fail), deformations measured at 5, 10, 25, and 50% of the failure load (D₅, D₁₀, D₂₅, and D₅₀, respectively), and stiffness measured at 50% of the failure force (K₅₀). There were no significant differences in the values for F_fail, D_fail, and E_fail between the experimental and control ligaments (p > 0.33). In contrast, the deformation values were all larger for the experimental than the control ligaments (p < 0.01). The deformations D₅, D₁₀, D₂₅, and D₅₀ (mean ± SD) for the control were 0.36 ± 0.13, 0.49 ± 0.23, 0.81 ± 0.35, and 1.23 ± 0.41 mm. The corresponding deformations for the experimental ligaments were, respectively, 209, 186, 153, and 130% of the control values. K₅₀ was also greater for the experimental ligament (125.0 ± 41.7 N/mm compared with 108.7 ± 31.4 N/mm, p < 0.03). These findings indicate that even though the strength of the ligament did not change due to a subfailure injury, the shape of the load-displacement curve, especially at low loads, was significantly altered. Under the dynamic in vivo loading conditions of daily living, this may result in increased joint laxity, additional loads being applied to other joint structures, and, with time, to joint problems.