Undecalcified preparation of bone tissue: report of technical experience and development of new methods

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Hahn, Michael¹; Vogel, Martin¹; Delling, Günter¹

Undecalcified preparation of bone tissue: report of technical experience and development of new methods

Virchows Archiv A. January 1991;418(1):1-7

©1991 Springer-Verlag

Affiliations

¹Department of Bone Pathology, Institute of Pathology, University of Hamburg, Hamburg 20, Federal Republic of Germany
Abstract and keywords

For reliable quantitative and qualitative analysis of bone specimens undecalcified preparation is essential. The “conventional” technique for this purpose is embedding in methylmethacrylate. Larger bone specimens, highly sclerotic specimens, cortical bone or bone implants consisting of metals or ceramics require modifications of this technique or completely new methods. We report our experience with the undecalcified preparation of 47700 bone specimens. New techniques such as the cutting of large area sections up to a size of 5×6 cm and grinding procedures for completely artefact-free preparation which are applied in special cases are also described. A new technique of combinded two- and three-dimensional analysis of bone specimens is presented. In our experience these methods are fundamental for morphological investigation of bone.

Keywords: Bone; Undecalcified preparation; Grinding technique
Links

DOI: 10.1007/BF01600238

PubMed: 1899163

WoS: A1991ET30700001
History

Received: 1990-07-05

Accepted: 1990-09-24
Cited Works (1)

Year Entry

1983 Recker RR, ed. Bone Histomorphometry: Techniques and Interpretation. Boca Raton, FL: Inc., CRC Press; 1983.

Cited By (19)

Year	Entry
2005	Uhrenholt L, Nielsen E, Vesterby Charles A, Gregersen M, Melsen F. Examination of the lower cervical spine facet joints in a road traffic crash fatality: a case study. In: Proceedings of the 2005 International IRCOBI Conference on the Biomechanics of Impact. September 21-23, 2005; Prague, Czech Republic.411-414.
2010	Busse B, Djonic D, Milovanovic P, Hahn M, Püschel K, Ritchie RO, Djuric M, Amling M. Decrease in the osteocyte lacunar density accompanied by hypermineralized lacunar occlusion reveals failure and delay of remodeling in aged human bone. Aging Cell. December 2010;9(6):1065-1075.
1996	Amling M, Pösl M, Ritzel H, Hahn M, Vogel M, Wening VJ, Delling G. Architecture and distribution of cancellous bone yield vertebral fracture clues: a histomorphometric analysis of the complete spinal column from 40 autopsy specimens. Arch Orthop Trauma Surg. July 1996;115(5):262-269.
2015	Milovanovic P, Zimmermann EA, Riedel C, vom Scheidt A, Herzog L, Krause M, Djonic D, Djuric M, Püschel K, Amling M, Ritchie RO, Busse B. Multi-level characterization of human femoral cortices and their underlying osteocyte network reveal trends in quality of young, aged, osteoporotic and antiresorptive-treated bone. Biomaterials. March 2015;45:46-55.
1992	Hahn M, Vogel M, Pompesius-Kempa M, Delling G. Trabecular bone pattern factor: a new parameter for simple quantification of bone microarchitecture. Bone. 1992;13(4):327-330.
1995	Grote HJ, Amling M, Vogel M, Hahn M, Pösl M, Delling G. Intervertebral variation in trabecular microarchitecture throughout the normal spine in relation to age. Bone. March 1995;16(3):301-308.
1996	Müller R, Hahn M, Vogel M, Delling G, Rüegsegger P. Morphometric analysis of noninvasively assessed bone biopsies: comparison of high-resolution computed tomography and histologic sections. Bone. March 1996;18(3):215-220.
2002	Lochmüller E-M, Groll O, Kuhn V, Eckstein F. Mechanical strength of the proximal femur as predicted from geometric and densitometric bone properties at the lower limb versus the distal radius. Bone. January 2002;30(1):207-216.
2009	Busse B, Hahn M, Soltau M, Zustin J, Püschel K, Duda GN, Amling M. Increased calcium content and inhomogeneity of mineralization render bone toughness in osteoporosis: mineralization, morphology and biomechanics of human single trabeculae. Bone. December 2009;45(6):1034-1043.
2014	Carriero A, Doube M, Vogt M, Busse B, Zustin J, Levchuk A, Schneider P, Müller R, Shefelbine SJ. Altered lacunar and vascular porosity in osteogenesis imperfecta mouse bone as revealed by synchrotron tomography contributes to bone fragility. Bone. April 2014;61:116-124.
2018	Milovanovic P, vom Scheidt A, Mletzko K, Sarau G, Püschel K, Djuric M, Amling M, Christiansen S, Busse B. Bone tissue aging affects mineralization of cement lines. Bone. May 2018;110:187-193.
2021	Köhli P, Appelt J, Otto E, Jahn D, Baranowsky A, Bahn A, Erdmann C, Müchler J, Mülleder M, Tsitsilonis S, Keller J. Effects of CGRP receptor antagonism on glucose and bone metabolism in mice with diet-induced obesity. Bone. February 2021;143:115646.
1993	Engelke K, Graeff W, Meiss L, Hahn M, Delling G. High spatial resolution imaging of bone mineral using computed microtomography: comparison with microradiography and undecalcified histologic sections. Invest Radiol. April 1993;28(4):341-349.
1995	Hahn M, Vogel M, Amling M, Ritzel H, Delling G. Microcallus formations of the cancellous bone: a quantitative analysis of the human spine. J Bone Miner Res. September 1995;10(9):1410-1416.
1996	Amling M, Herden S, Pösl M, Hahn M, Ritzel H, Delling G. Heterogeneity of the skeleton: comparison of the trabecular microarchitecture of the spine, the iliac crest, the femur, and the calcaneus. J Bone Miner Res. January 1996;11(1):36-45.
1997	Ritzel H, Amling M, Pösl M, Hahn M, Delling G. The thickness of human vertebral cortical bone and its changes in aging and osteoporosis: a histomorphometric analysis of the complete spinal column from thirty‐seven autopsy specimens. J Bone Miner Res. January 1997;12(1):89-95.
2004	Eckstein F, Wunderer C, Boehm H, Kuhn V, Priemel M, Link TM, Lochmüller E-M. Reproducibility and side differences of mechanical tests for determining the structural strength of the proximal femur. J Bone Miner Res. March 2004;19(3):379-385.
2020	Ries C, Boese CK, Stürznickel J, Koehne T, Hubert J, Pastor M-F, Hahn M, Meier SL, Beil FT, Püschel K, Amling M, Rolvien T. Age-related changes of micro-morphological subchondral bone properties in the healthy femoral head. Osteoarthritis Cartilage. November 2020;28(11):1437-1447.
2002	Egerer AK. The Relationship Between the Osteonal Cement Line and Bone Strength [PhD thesis]. Loma Linda University; June 2002.