Whole-body vibration and muscle fatigue have both been shown to delay the trunk muscle reflex response and increase trunk muscle activation, leading to an increased risk of low back injuries. However, the effects of whole-body vibration on previously fatigued trunk muscles have never been tested, despite tests showing that prolonged exposure to wholebody vibration can lead to muscle fatigue. The purpose of this research was to investigate the effects of muscle fatigue on muscle latency, muscle activation and perceived discomfort when exposed to whole-body vibration. The results showed that a fatigued muscle state resulted in increased muscle latency, muscle activation and perceived discomfort, which all escalate the risk of low back injuries. Additionally, the ISO 2631-1 comfort ratings did not increase with fatigue, showing a disconnect between these comfort ratings and the perceived discomfort ratings in a fatigued muscle state.