Infrared spectroscopy, microscopy, and microscopic imaging of mineralizing tissues:​ spectra-structure correlations from human iliac crest biopsies

Mendelsohn, Richard; Paschalis, Eleftherios P.; Boskey, Adele L.

Affiliations

¹Rutgers University, Department of Chemistry, Newark, New Jersey 07102

²The Hospital for Special Surgery, Mineralized Tissue Section, Research Division, New York, New York 10021

Abstract and keywords

Infrared microscopic images of the cortical region of human iliac crest biopsies have been obtained at ~7 μm spatial resolution and 8 cm⁻¹ spectral resolution with a 64×64 mercury–cadmium–telluride focal plane array detector coupled to a Fourier transform infrared microscope and a step scanning interferometer. Images of several spectral parameters provide information about the spatial distribution of the mineral (apatite) and protein (mostly collagen) components of the tissue. In addition, the image of a parameter known to reflect the crystallinity/perfection of the mineral phase, namely, the intensity ratio of bands at 1030 and 1020 cm⁻¹ within the phosphate ν₁, ν₃ contour, revealed a progressive increase in the apatite crystal size/perfection from the osteonal center to the periphery. Finally, a detailed comparison of the spatial distribution of the I(1020)/I(1030) ratio for the same osteon obtained by array detection and by conventional point-by-point microspectroscopy revealed statistically identical behavior, thereby providing a validation of infrared imag- ing for structural analysis of apatite forming tissues.

Keywords: vibrational imaging; array detection; biomineralization; hydroxyapatite; collagen

Links

DOI: 10.1117/1.429916

PubMed: 23015164

WoS: 000082183900003

History

Received: 1998-06-25

Revised: 1998-10-22

Accepted: 1998-10-30

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Cited By (9)

Year	Entry
2021	Gamsjaeger S, Fratzl P, Paschalis EP. Interplay between mineral crystallinity and mineral accumulation in health and postmenopausal osteoporosis. Acta Biomater. April 2021;124:374.
2002	Aparicio S, Doty SB, Camacho NP, Paschalis EP, Spevak L, Mendelsohn R, Boskey AL. Optimal methods for processing mineralized tissues for Fourier transform infrared microspectroscopy. Calcif Tiss Int. May 2002;70(5):422-429.
2002	Boskey AL, Spevak L, Paschalis E, Doty SB, McKee MD. Osteopontin deficiency increases mineral content and mineral crystallinity in mouse bone. Calcif Tiss Int. August 2002;71(2):145-154.
2003	Paschalis EP, Burr DB, Mendelsohn R, Hock JM, Boskey AL. Bone mineral and collagen quality in humeri of ovariectomized cynomolgus monkeys given rhPTH(1–34) for 18 months. J Bone Miner Res. April 2003;18(4):769-775.
2003	Paschalis EP, Boskey AL, Kassem M, Eriksen EF. Effect of hormone replacement therapy on bone quality in early postmenopausal women. J Bone Miner Res. June 2003;18(6):955-959.
2003	Paschalis E, Recker R, Dicarlo E, Doty S, Atti E, Boskey A. Distribution of collagen cross-links in normal human trabecular bone. J Bone Miner Res. November 2003;18(11):1942-1946.
2017	Unal M. Classification of Bound Water and Collagen Denaturation Status of Cortical Bone by Raman Spectroscopy [PhD thesis]. Cleveland, OH: Case Western Reserve University; January 2017.
2020	Taylor EA. Validation and Implementation of Vibrational Spectroscopic Measures of Bone Composition [PhD thesis]. Ithaca, NY: Cornell University; December 2020.
2013	Poundarik A. The Role of Non-Collagenous Proteins, in Particular, Osteocalcin and Osteopontin, in the Determination of Bone Matrix Quality [PhD thesis]. Troy, NY: Rensselaer Polytechnic Institute; May 2013.