Two way cycle lanes are common in Germany especially in rural areas. However, sometimes they are also used within city limits. There are special accident risks connected with two way cycle paths. These are in towns at crossings where especially the drivers of motorised vehicles often do not consider cyclists using the left side cycle path and in rural areas at points where the cyclists need to cross the main road in order to get to the cycle path. For this study data from GIDAS (German In Depths Accident Study) is analysed which is based on a representative accident sample that is collected in the cities and surrounding areas of Dresden and Hannover. It is important to know that in the town of Hannover cycle paths are considerably often designed as two way cycle paths while this is not the case in Dresden. There are about 1,500 cyclists in the GIDAS sample that were involved in an accident using the left cycle path inside city limits and about 50 outside city limits. Both groups are almost similar in size compared to the cyclists that are using the cycle path on the right side. This paper analyses accident risks for cyclists using the left cycle path compared to those using the right one. There is also a comparison between those that used the left cycle path where it was allowed to those that used the left cycle path where it was not allowed. The analysis is conducted also by comparing the Dresden data to the Hannover data in order to analyse whether or not there are differences for the two cities with different cycle path design policies. In the scope of this study scenarios were created for conflicts of vehicles with cyclists at junctions in towns, while accidents in rural areas where the cyclists need to cross the main road due to only one available cycle path could not be identified efficiently enough from the database. In general for the accidents at junctions in towns the analysis revealed that accidents with cyclists travelling on the left bicycle path (allowed or not) are not prone to a higher accident risk where a road user turns off the main road. There is however a higher risk at junctions where a road user crosses the bicycle path to enter the priority road which is influenced by the observation strategy of the entering vehicle. The comparison of the accident situations of the cities Dresden and Hannover also revealed that seemingly there is no “training effect” in Hannover meaning that the road users there could be more used to bicyclists on the left cycle path.