Fore-and-aft vibration of a backrest can influence discomfort and the risk of injury associated with whole-body vibration. Relevant standards (BS 6841:​1987 and ISO2631-1:​1997) recommend the W_c frequency weighting for evaluating fore-and-aft vibration of backrests, but do not specify the precise location for measuring vibration. This study determined equivalent comfort contours for fore-and-aft vibration of the backs of seated persons from 2 to 80 Hz using the method of magnitude estimation, examining the effect of input location, contact area, and body posture. The equivalent comfort contours indicate decreased sensitivity to vibration acceleration at frequencies greater than 8 Hz. Equivalent comfort contours with a full backrest were similar to those with contact at only the highest location on the back. The derived frequency weightings are broadly consistent with frequency weighting W_c but suggest somewhat greater sensitivity at frequencies greater than 30 Hz and vary in shape with changes in vibration magnitude. It is concluded that with low and moderate magnitudes of vibration the severity of fore-and-aft vibration of a backrest can be assessed from the frequency-weighted fore-and-aft acceleration measured at the highest point of contact between the backrest and the body if the frequency weighting W c is employed in the evaluation.