Study Design: A uniaxial tensile loading study of 13 lumbar porcine ligaments under varying environmental temperature conditions.
Objectives: To investigate a possible temperature dependence of the material behavior of porcine lumbar anterior longitudinal ligaments.
Summary of Background Data: Temperature dependence of the mechanical material properties of ligament has not been conclusively established.
Methods: The anterior longitudinal ligaments (ALLs) from domestic pigs (n = 5) were loaded in tension to 20% strain using a protocol that included fast ramp/hold and sinusoidal tests. These ligaments were tested at temperatures of 37.8°C, 29.4°C, 21.1°C, 12.8°C, and 4.4°C. The temperatures were controlled to within 0.6°C, and ligament hydration was maintained with a humidifier inside the test chamber and by spraying 0.9% saline onto the ligament. A viscoelastic model was used to characterize the force response of the ligaments.
Results: The testing indicated that the ALL has strong temperature dependence. As temperature decreased, the peak forces increased for similar input peak strains and strain rates. The relaxation of the ligaments was similar at each temperature and showed only weak temperature dependence. Predicted behavior using the viscoelastic model compared well with the actual data (R2 values ranging from 0.89 to 0.99). A regression analysis performed on the viscoelastic model coefficients confirmed that relaxation coefficients were only weakly temperature dependent while the instantaneous elastic function coefficients were strongly temperature dependent.
Conclusions: The experiment demonstrated that the viscoelastic mechanical response of the porcine ligament is dependent on the temperature at which it is tested; the force response of the ligament increased as the temperature decreased. This conclusion also applies to human ligaments owing to material and structural similarity. This result settles a controversy on the temperature dependence of ligament in the available literature. The ligament viscoelastic model shows a significant temperature dependence on the material properties; instantaneous elastic force was clearly temperature dependent while the relaxation response was only weakly temperature dependent. This result suggests that temperature dependence should be considered when testing ligaments and developing material models for in vivo force response, and further suggests that previously published material property values derived from room temperature testing may not adequately represent in vivo response. These findings have clinical relevance in the increased susceptibility of ligamentous injury in the cold and in assessing the mechanical behavior of cold extremities and extremities with limited vascular perfusion such as those of the elderly.
|1992||Pintar FA, Yoganandan N, Myers T, Elhagediab A, Sances A Jr. Biomechanical properties of human lumbar spine ligaments. J Biomech. November 1992;25(11):1351-1356.|
|1990||Lam TC, Thomas CG, Shrive NG, Frank CB, Sabiston CP. The effects of temperature on the viscoelastic properties of the rabbit medial collateral ligament. J Biomech Eng. May 1990;112(2):147-152.|
|1981||Woo SL-Y, Gomez MA, Akeson WH. The time and history-dependent viscoelastic properties of the canine medical collateral ligament. J Biomech Eng. November 1981;103(4):293-298.|
|1986||Woo SL-Y, Orlando CA, Camp JF, Akeson WH. Effects of postmortem storage by freezing on ligament tensile behavior. J Biomech. 1986;19(5):399-404.|
|1987||Woo SL-Y, Lee TQ, Gomez MA, Sato S, Field FP. Temperature dependent behavior of the canine medial collateral ligament. J Biomech Eng. February 1987;109(1):68-71.|
|2009||Santago A, Kemper A, McNally C, Sparks J, Duma S. The effect of temperature on the mechanical properties of bovine liver. In: Biomedical Sciences Instrumentation. Vol 45. 2009:376-381.|
|2015||Stemper BD, Pintar FA, Baisden JL. Lumbar spine injury biomechanics. In: Yoganandan N, Nahum AM, Melvin JW, eds. Accidental Injury: Biomechanics and Prevention. 3rd ed. New York: Springer; 2015:451-470.|
|2019||Op ‘t Eynde J, Eckersley CP, Bass CR. High-rate viscoelastic shear model of porcine skin, lung and liver tissue. In: Proceedings of the 2019 International IRCOBI Conference on the Biomechanics of Injury. September 11-13, 2019; Florence, Italy.475.|
|2012||Mattucci SFE, Moulton JA, Chandrashekar N, Cronin DS. Strain rate dependent properties of younger human cervical spine ligaments. J Mech Behav Biomed Mater. June 2012;10:216-226.|
|2013||Frimenko RE, Lievers WB, Riley PO, Park JS, Hogan MV, Crandall JR, Kent RW. Development of an injury risk function for first metatarsophalangeal joint sprains. Med Sci Sports Exer. November 2013;45(11):2144-2150.|
|2013||Lu Y-C, Untaroiu CD. Effect of storage methods on indentation-based material properties of abdominal organs. Proc Inst Mech Eng Part H-J Eng Med. March 2013;227(3):293-301.|
|2013||Jamison D IV. Mechanical Characterization of the Human Lumbar Intervertebral Disc Subjected to Impact Loading Conditions [PhD thesis]. Drexel University; August 2013.|
|2015||Wood GW. Interspecies Scaling in Blast Neurotrauma [PhD thesis]. Duke University; 2015.|
|2009||Forman J. The Structural Characteristics of the Costal Cartilage: The Roles of Calcification and the Perichondrium, and the Representation of the Costal Cartilage in Finite Element Models of the Human Body [PhD thesis]. Charlottesville, VA: University of Virginia; August 2009.|
|2013||Frimenko RE. Injury Mechanism and Threshold of Acute First Metatarsophalangeal Joint Sprains [Master's thesis]. Charlottesville, VA: University of Virginia; May 2013.|
|2011||Howarth SJ. Mechanical Response of the Porcine Cervical Spine to Acute and Repetitive Anterior-Posterior Shear [PhD thesis]. University of Waterloo; 2011.|
|2011||Mattucci S. Strain Rate Dependent Properties of Younger Human Cervical Spine Ligaments [Master's thesis]. Waterloo, ON: University of Waterloo; 2011.|