Finite element models of the lumbar spine often adopt ligament properties from tensile tests without accounting for possible differences between testing and in situ initial ligament length. Such differences could result in laxities or preloads at the beginning of a simulation that would affect the ligament forces, tangent stiffness, and the posture at which they fail. In vivo and in vitro human experimental data reported laxities or preloads. However, laxities or preloads, which could also result from postural differences, are often neglected in simulation studies. This study proposes a numerical methodology to identify ranges of ligament laxities or preloads compatible with the selected tensile ligament properties, the model, and the range of motion (RoM) the model aims to simulate. The approach assumes that ligaments should remain in a safe elongation range for the complete RoM, and that each ligament should play a significant mechanical role in at least one load case. The methodology was applied to the functional spinal unit (FSU) models using the RoM from healthy subjects and ligament properties from the literature. Without laxity, some ligaments reached their elongation at failure within the RoM. Laxity ranges varied considerably (from −9.2 mm preload to 10.7 mm laxity) and flexion was the most critical load case to determine them. Their effect on the mobility response was also assessed. The effect on the mobility of a FSU was also assessed. While the proposed method cannot determine an exact laxity value, it is simple and it can be applied to any model to identify a plausible range of ligament initial length.