Quantitative structural organization of normal adult human articular cartilage

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Hunziker, E. B.¹; Quinn, T. M.¹; Häuselmann, H.-J.¹

Quantitative structural organization of normal adult human articular cartilage

Osteoarthritis Cartilage. July 2002;10(7):564-572

©2002 OsteoArthritis Research Society International. Published by Elsevier Science Ltd. All rights reserved.

Affiliations

¹M. E. Müller-Institute for Biomechanics, University of Bern, Murtenstrasse 35, P.O. Box 30, CH-3010 Bern, Switzerland
Abstract and keywords

Objective: Data pertaining to the quantitative structural features and organization of normal articular cartilage are of great importance in understanding its biomechanical properties and in attempting to establish this tissue’s counterpart by engineering in vitro. A comprehensive set of such baseline data is, however, not available for humans. It was the purpose of the present study to furnish the necessary information.

Design: The articular cartilage layer covering the medial femoral condyle of deceased persons aged between 23 and 49 years was chosen for the morphometric analysis of cell parameters using confocal microscopy in conjunction with unbiased stereological methods. The height of the hyaline articular cartilage layer, as well as that of the calcified cartilage layer and the subchondral bone plate, were also measured.

Results: The mean height of the hyaline articular cartilage layer was found to be 2.4 mm, the volume density of chondrocytes therein being 1.65%, the number of cells per mm³ of tissue 9626 and the mean cell diameter 13 μm. Other estimators (including matrix mass per cell and cell profile density) were also determined.

Conclusions: A comparison of these normal human quantitative data with those published for experimental animals commonly used in orthopaedic research reveals substantial differences, consideration of which in tissue engineering strategies destined for human application are of paramount importance for successful repair. © 2002 OsteoArthritis Research Society International. Published by Elsevier Science Ltd. All rights reserved.

Keywords: Human; Articular; Cartilage; Morphometry
Links

DOI: 10.1053/joca.2002.0814

PubMed: 12127837

WoS: 000177625700009
History

Received: 2002-01-25

Accepted: 2002-04-08

Cited Works (10)

Year	Entry
1999	Hunziker EB. Articular cartilage repair: are the intrinsic biological constraints undermining this process insuperable? Osteoarthritis Cartilage. January 1999;7(1):15-28.
1997	Poole CA. Articular cartilage chondrons: form, function and failure. J Anat. 1997;191(1):1-13.
1988	Eggli PS, Hunzinker EB, Schenk RK. Quantitation of structural features characterizing weight- and less-weight-bearing regions in articular cartilage: a stereological analysis of medical femoral condyles in young adult rabbits. Anat Rec. November 1988;222(3):217-227.
1993	Mow VC, Ateshian GA, Spilker RL. Biomechanics of diarthrodial joints: a review of twenty years of progress. J Biomech Eng. November 1993;115(4B):460-467.
1999	Shepherd DET, Seedhom BB. Thickness of human articular cartilage in joints of the lower limb. Ann Rheum Dis. January 1999;58(1):27-34.
1988	Gundersen HJG, Bendtsen TF, Korbo L, Marcussen N, Møller A, Nielsen K, Nyengaard JR, Pakkenberg B, Sørensen FB, Vesterby A, West MJ. Some new, simple and efficient stereological methods and their use in pathological research and diagnosis. AMPIS. May 1988;96(5):379-394.
1998	Vunjak-Novakovic G, Obradovic B, Martin I, Bursac PM, Langer R, Freed LE. Dynamic cell seeding of polymer scaffolds for cartilage tissue engineering. Biotechnol Prog. March–April 1998;14(2):193-202.
1968	Weiss C, Rosenberg L, Helfet AJ. An ultrastructural study of normal young adult human articular cartilage. J Bone Joint Surg. June 1968;50A(4):663-674.
1997	Buckwalter JA, Mankin HJ. Articular cartilage, I: tissue design and chondrocyte-matrix interactions. J Bone Joint Surg. April 1997;79A(4):600-611.
1996	Wong M, Wuethrich P, Eggli P, Hunziker E. Zone-specific cell biosynthetic activity in mature bovine articular cartilage: a new method using confocal microscopic stereology and quantitative autoradiography. J Orthop Res. May 1996;14(3):424-432.

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2005	Jadin KD, Wong BL, Bae WC, Li KW, Williamson AK, Schumacher BL, Price JH, Sah RL. Depth-varying density and organization of chondrocytes in immature and mature bovine articular cartilage assessed by 3D imaging and analysis. J Histochem Cytochem. September 2005;53(9):1109-1119.
2020	Wang Q, Tang T, Cooper D, Eltit F, Fratzl P, Guy P, Wang R. Globular structure of the hypermineralized tissue in human femoral neck. J Struct Biol. November 1, 2020;212(2):107606.
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2014	Motavalli SM. Depth-Dependent Biaxial Mechanical Behavior of Native and Tissue Engineering Articular Cartilage [PhD thesis]. Cleveland, OH: Case Western Reserve University; May 2014.
2017	Wahlquist JA. Zonal Articular Cartilage Possesses Complex Mechanical Behavior Spanning Multiple Length Scales: Dependence on Chemical Heterogeneity, Anisotropy, and Microstructure [PhD thesis]. Boulder, CO: University of Colorado; 2017.
2007	Jiang J. The Role of Cellular Interactions in Osteochondral Graft Design and the Maintenance of a Stable Osteochondral Interface [PhD thesis]. Columbia University; 2007.
2012	Khanarian NT. Scaffold Design and Optimization for Osteochondral Interface Tissue Engineering [PhD thesis]. Columbia University; 2012.
2016	Boushell MK. Scaffold Design and Optimization for Integrative Cartilage Repair [PhD thesis]. Columbia University; 2016.
2020	Mosher CZ. Interface Scaffold Design Principles for Integrative Cartilage Regeneration [PhD thesis]. Columbia University; 2020.
2007	Leddy HA. Diffusional Properties of Articular Cartilage [PhD thesis]. Duke University; 2007.
2009	Christensen SE. The Role of Collagen VI in the Structure and Properties of the Knee Joint [PhD thesis]. Duke University; 2009.
2013	Wilusz RE. Micromechanical Properties of the Extracellular and Pericellular Matrices of Articular Cartilage [PhD thesis]. Duke University; 2013.
2020	Kessel B. Advanced Biofabrication Methods for Cartilage Tissue Engineering [PhD thesis]. Swiss Federal Institute of Technology Zürich; 2020.
2020	Levinson CSJ. From Bedside to Bench and Back: Translatable Approaches for Cartilage Repair [PhD thesis]. Swiss Federal Institute of Technology Zürich; 2020.
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2011	Changoor A. Electromechanical and Polarization Microscopy Evaluation of Cartilage Repair and Degeneration [PhD thesis]. École polytechnique de Montréal; January 2011.
2017	Dwivedi G. Identification of in Situ Progenitor Cell Features Contributing to Cartilage Repair and Strategies for Cartilage Repair Augmentation [PhD thesis]. École polytechnique de Montréal; April 2017.
2017	Sim S. Développement d'une base de données cartographiques et de modèles statistiques pour la caractérisation du cartilage articulaire [PhD thesis]. École polytechnique de Montréal; May 2017.
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2009	McGregor AJ. A Method to Improve Cartilage Integration [Master's thesis]. Queen's University; December 2009.
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2011	Jeon JE. Development of Zonal Cartilage Constructs: Effects of Chondrocyte Subpopulation, Compressive Stimulation, and Culture Models [PhD thesis]. Queensland University of Technology; 2011.
2015	Kuaha K. Environments for Zonal Cartilage Tissue Engineering [PhD thesis]. Queensland University of Technology; 2015.
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2011	Bourne DA. Chondrocyte Viability After in Vivo Muscular and Impact Loading [PhD thesis]. Calgary, AB: University of Calgary; May 2011.
2014	Jablonski CL. Integrin Α1β1 Participates in Chondrocyte Transduction of Osmotic Stress [Master's thesis]. Calgary, AB: University of Calgary; May 2014.
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2006	Jadin KD. Mechanisms of Growth of Articular Cartilage: Cell Organization and Fates [PhD thesis]. San Diego (UCSD), University of California; 2006.
2006	Nugent GE. Biomechanical Regulation of Articular Cartilage Metabolism of Proteoglycan 4 and Articular Surface Integrity [PhD thesis]. San Diego (UCSD), University of California; 2006.
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