Some new, simple and efficient stereological methods and their use in pathological research and diagnosis

Gundersen, H. J. G.; Bendtsen, T. F.; Korbo, L.; Marcussen, N.; Møller, A.; Nielsen, K.; Nyengaard, J. R.; Pakkenberg, B.; Sørensen, F. B.; Vesterby, A.; West, M. J.

Affiliations

¹Stereological Research Laboratory, University Institute of Pathology and 2nd University Clinic of Internal Medicine, Institute of Experimental Clinical Research, University of Århus

²Neurological Research Laboratory, Hvidovre Hospital

³Institute of Pathology, University of Copenhagen, Rigshospitalet

⁴University Institute of Pathology, Århus Amtsygehus, Denmark

Abstract and keywords

Stereology is a set of simple and efficient methods for quantitation of three-dimensional microscopic structures which is specifically tuned to provide reliable data from sections. Within the last few years, a number of new methods has been developed which are of special interest to pathologists. Methods for estimating the volume, surface area and length of any structure are described in this review. The principles on which stereology is based and the necessary sampling procedures are described and illustrated with examples. The necessary equipment, the measurements, and the calculations are invariably simple and easy.

Keywords: Cavalieri’s principle; isotropic sections; star volume; surface area; stereology; vertical sections; volume

Links

DOI: 10.1111/j.1699-0463.1988.tb05320.x

PubMed: 3288247

WoS: A1988N950400001

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2021	Klose-Jensen R, Hartlev LB, Thomsen JS, Nyengaard JR, Boel LWT, Laursen M, Laurberg TB, Stengaard-Pedersen K, Hauge E-M. Calcified cartilage in patients with osteoarthritis of the hip compared to that of healthy subjects: a design-based histological study. Bone. February 2021;143:115660.
2022	Lamarche BA, Thomsen JS, Andreasen CM, Lievers WB, Andersen TL. 2D size of trabecular bone structure units (BSU) correlate more strongly with 3D architectural parameters than age in human vertebrae. Bone. July 2022;160:116399.
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