Mechanotransduction in stretched osteocytes: temporal expression of immediate early and other genes

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Kawata, Akira¹; Mikuni-Takagaki, Yuko¹

Mechanotransduction in stretched osteocytes: temporal expression of immediate early and other genes

Biochem Biophys Res Commun. May 19, 1998;246(2):404-408

©1998 Academic Press.

Affiliations

¹Departments of Oral Biochemistry, Kanagawa Dental College, Yokosuka, 238-8580, Japan
Abstract

Osteocytes, dendritic bone cells, transduce signals of mechanical loading that results in bone formation. We have reported in stretched primary osteocytes that the cAMP level, IGF-I and osteocalcin protein levels were elevated (Endocrinology 137:2028, 1996). Here we report that stretching induces the expression of immediate early genes, c-fos and COX-2; inducive cyclooxygenase gene. Compared to c-fos, COX-2 as well as IGF-I and osteocalcin mRNA appeared in a biphasic manner; second peaks at 8 (COX-2) or 24 hrs (IGF-I and osteocalcin) later. Furthermore, these second peaks are abolished by including NS398, a specific inhibitor of the inducive cyclooxygenase, during the 3-hr stretching. A sequence that the calcium influx activates PkA which, in turn, activates c-fos and COX-2 transcription resulting in the production of proteins such as IGF-I and osteocalcin. A long-lasting effect of mechanical loadingin vivocan be explained from the secondary anabolic reaction we observed through the upregulated COX-2 mRNA.
Links

DOI: 10.1006/bbrc.1998.8632

PubMed: 9610372

WoS: 000073796900020
History

Received: 1998-04-1

Cited Works (7)

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2003	Noble BS, Peet N, Stevens HY, Brabbs A, Mosley JR, Reilly GC, Reeve J, Skerry TM, Lanyon LE. Mechanical loading: biphasic osteocyte survival and targeting of osteoclasts for bone destruction in rat cortical bone. Am J Physiol Cell Physiol. April 2003;284(4):C934-C943.
2021	Smit TH. Closing the osteon: do osteocytes sense strain rate rather than fluid flow? BioEssays. August 2021;43(8):2000327.
2003	Simmons CA, Matlis S, Thornton AJ, Chen S, Wang C-Y, Mooney DJ. Cyclic strain enhances matrix mineralization by adult human mesenchymal stem cells via the extracellular signal-regulated kinase (ERK1/2) signaling pathway. J Biomech. August 2003;36(8):1087-1096.
2004	Mullender M, El Haj AJ, Yang Y, van Duin MA, Burger EH, Klein-Nulend J. Mechanotransduction of bone cells in vitro: mechanobiology of bone tissue. Med Biol Eng Comput. January 2004;42(1):14-21.
2002	Ehrlich PJ, Lanyon LE. Mechanical strain and bone cell function: a review. Osteoporos Int. September 2002;13(9):688-700.
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2009	Chan M. Mechanobiology of 3D Co-Culture Trabecular Explant Model [PhD thesis]. Columbia University; 2009.
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2003	Ominsky MS. Effects of Hydrostatic Pressure, Biaxial Strain, and Fluid Shear on Osteoblastic Cells: Mechanotransduction Via NF-ΚB, MAP Kinase, and AP-1 Pathways [PhD thesis]. University of Michigan; 2003.
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