Organ-on-a-chip technologies have made significant strides in recapitulating the complex multicellular 3D architectures, tissue-tissue interfaces and the physiologically relevant mechanical and biological properties of in vivo tissue microenvironments. To accelerate current drug development processes, new and improved in vitro models that reconstitute the complex in vivo microenvironment of human tissues are needed. Local disruptions to the lung airway epithelium are known to elicit specific cellular responses leading to the progression of a myriad of respiratory diseases, making it a crucial target for conducting disease mechanism and drug development studies. We aimed to develop and perform preliminary assessment on a microfluidic-based in vitro model that mimics the in vivo tissue microenvironments of the human airway. By leveraging microfabrication techniques and surface tension principles to integrate co-culture of airway cells on stable air- liquid interfaces, we demonstrated a microfluidic platform that allows more efficient 3D culture experiments and enables high-throughput co-culture studies of the airway tissue.