Ensuring human health, safety, comfort, and productivity is a key factor in the management of both construction and built environments (CBEs);​ human workers are the most important resource at construction sites and the operation of most built environments places the highest priority on serving people optimally. However, the “one-size-fits-all” approach, widely applied in current CBE management practices, is not effective for serving humans in CBEs because every individual has unique characteristics and thus differently interacts with CBEs even under an identical setup. Wearable biosensors have great potential to continuously and less-invasively monitor stress as the indicator of individuals’ quality of experience during their daily work and lives, thereby enabling more individual response-aware CBE management. However, still there is a lack of fieldapplicable means (1) to detect stress from biosignals in an noise-robust and scalable manner;​ and (2) to provide stress cues (e.g., location and impact of stress cases) useful in understanding related stressors and designing effective interventions, despite these means’ necessity in realizing the wearable biosensors’ potential in CBEs. To fill these gaps, five interrelated studies were conducted (1) to denoise both stationary and non-stationary artifacts in biosignals collected during people’s daily work and lives in CBEs;​ (2) to reliably assess generalizability of machine learning models for tasks monitoring human responses from biosignals;​ (3) to advance model generalizability across different subjects and contexts in detecting stress using a wearable biosensor;​ (4) to distinguish and locate stress responses related to environmental features;​ and (5) to differentiate stress types into positive (i.e., eustress) and negative types (i.e., distress) in terms of their impact on individuals. These studies can be the first steppingstones for operating and managing CBEs while actively accounting for individuals’ responses. Such individual response-aware CBE operations will significantly contribute to improving human safety, health, and comfort in CBEs and ultimately promoting not only the performance of the construction industry, but only people’s quality of life in built environments.