The development of bacterial biofilm is central to the pathogenesis of periprosthetic joint infections following arthroplasty. Consequently, biofilm research in orthopedic surgery has expanded. This study assessed the quality of current orthopedic literature pertaining to the study of biofilm and identified the most commonly used study designs and techniques. A literature search was conducted in PubMed and adapted for Embase and the Cochrane Library. Studies were stratified into in vitro, in vivo (animal), and clinical (human) studies. Studies were included if they evaluated biofilm in an orthopedic context. Clinical studies were included if biofilm was confirmed by a quantification or visualization. Studies were assessed based on study design, biofilm quantification and visualization techniques and quality using the MIABiE score, a standardized tool in appraising biofilm studies. Of 258 studies identified;​ 65 studies were included after screening. There were 35 studies (50.7%) that evaluated techniques for biofilm treatment, 14 studies (20.3%) evaluated materials for prevention of biofilm formation, and 20 studies (29.0%) investigated descriptive biofilm properties unrelated to treatment or prevention. In vitro studies were most common (62.3%, n = 43). Biofilm quantification techniques were used in 83.0% of studies (n = 57), while only 29 studies (42.0%) used visualization techniques. Most studies (n = 55, 84.6%) were classified as low-quality based on MIABiE.