Despite extensive past lung cancer research, there still lacks an understanding of the relationship between the disease and its environmental factors, notably the cyclic mechanical stress induced by breathing. Here, a cell stretching platform, consisting of a polydimethylsiloxane (PDMS)-based cell culture device and a vacuum pump-based actuation system controlled and automated by an Arduino microcontroller was proposed to induce physiologically relevant levels of stretching stimuli on PC-9 human lung cancer cells. This system is inexpensive and easy to use, and ahs a novel membrane design that allows the platform to be miniaturized and operated in cell culture incubators. Results show cyclic stretching altered PC-9 cell morphology and reduced cell viability. Stretched cells also exhibited lower expressions of epithelial markers such as E-cadherin as well as higher expressions of mesenchymal markers and EMT transcription factors such as ZEB1/2 and Twist1/2. Stretching also led to increased cell motility due to the deterioration of vinculin.