A sensitive and cost-effective measurement of glucose has always been a priority in clinical and quality control arena. In this article, molecularly imprinted and non-imprinted polymer (MIP & NIP) based on conducting polymers and functionalized composites was suggested to achieve repeatable and stable determination of glucose in POC analyses. Glucose was introduced into the imprinted layer composed of polypyrrole (PPy) along with (APBA), which was formed on the carboxylayed multiwalled carbon nanotubes (COO-MWCNT) as the step to immobilize glucose. Further polymerization of an imprinting layer for glucose, in a basic medium, was performed to cage the glucose molecule. A layer-by-layer analysis of the sensor was performed with electrochemical and surface analyzing methods. Also, both NIP and MIP were characterized before and after the glucose removal by phosphate buffer. The glucose rebinding to form glucoboronate ester in sensor was tested using impedance technique and a linear range between 1 µM and 20 µM with the detection limit of 6 µM was achieved. The reusability and stability of the imprinted sensor were determined to be 10 times and 96% of the beginning current was maintained after 15 days. In overall, such a sensor demonstrates promising future for developing cheap, reusable and non-invasive glucose sensors.