Osteoarthritis (OA) is a degenerative joint disorder with the knee being the most common large joint affected by it. Knee OA has effects on gait patterns and these effects can change depending on the severity of the disease. Testing rehabilitation interventions for knee OA can provide an understanding of possible preventative measures. Animal models, such as the rat medial meniscal tear (MMT) model, have been used in testing interventions. Two interventions, exercise and immobilization, were applied to the rat MMT injury model. A custom biplanar X-ray video system was chosen to measure the kinematics of the experimental rats and quantify the effects of the interventions. Prior to use, workflows needed to be developed for this system. Additionally, the system needed to be validated and an appropriate analysis technique for the knee OA study needed to be chosen. An XMALab workflow reliant on manual recognition of joint centers and an Autoscoper workflow using 3D models of subject-specific bones were developed. The system’s accuracy and precision values were measured using phantoms of known length. Qualitative and quantitative differences between the two workflows were compared and the Autoscoper workflow was chosen for the knee OA study. Fourteen rats were organized into four experimental groups:​ a non-intervention, an exercise intervention, an immobilization intervention, and a sham surgery group. The kinematic and spatiotemporal parameters were measured at three gait cycle events. Hip abduction results indicated the non-intervention group developed mild OA, while hip abduction and knee varus rotation results indicated the exercise intervention group developed advanced OA. The immobilization group results were indistinguishable from atrophic changes.