In vivo measurement of human wrist extensor muscle sarcomere length changes

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Lieber, R. L.; Loren, G. J.; Fridén, J.

In vivo measurement of human wrist extensor muscle sarcomere length changes

J Neurophysiol. March 1994;71(3):874-881

©1994 The American Physiological Society

Affiliations

¹Department of Orthopaedics and Biomedical Sciences Graduate Group, University of California and Veterans Administration Medical Centers, San Diego, California 92161

²Departments of Anatomy and Hand Surgery, University of Umeä, S-90185 Umeä, Sweden
Abstract
1. Human extensor carpi radialis brevis (ECRB) sarcomere length was measured intraoperatively in five subjects using laser diffraction.
2. In a separate cadaveric study, ECRB tendons were loaded to the muscle's predicted maximum tetanic tension, and tendon strain was measured to estimate active sarcomere shortening at the expense of tendon lengthening.
3. As the wrist joint was passively flexed from full extension to full flexion, ECRB sarcomere length increased from 2.6 to 3.4 microns at a rate of 7.6 nm/deg joint angle rotation. Correcting for tendon elongation during muscle activation yielded an active sarcomere length range of 2.44 to 3.33 microns. Maximal predicted sarcomere shortening accompanying muscle activation was dependent on initial sarcomere length and was always < 0.15 microns, suggesting a minimal effect of tendon compliance.
4. Thin filament lengths measured from electron micrographs of muscle biopsies obtained from the same region of the ECRB muscles were 1.30 ± .027 (SE) microns whereas thick filaments were 1.66 ± .027 microns long, suggesting an optimal sarcomere length of 2.80 microns and a maximum sarcomere length for active force generation of 4.26 microns.
5. These experiments demonstrate that human skeletal muscles can function on the descending limb of their sarcomere length-tension relationship under physiological conditions. Thus, muscle force changes during joint rotation are an important component of the motor control system.
Links

DOI: 10.1152/jn.1994.71.3.874

PubMed: 8201427

WoS: A1994NA91600005
History

Received: 1993-08-10

Accepted: 1993-11-05

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