Skeletal fragility is a major health concern, resulting in deterioration in quality of life for those at risk and rising healthcare costs. The increasing prevalence of conditions known to affect bone fracture risk such as osteoporosis and diabetes, as well as the increasing aging of our population, contribute to this growing health issue. Among the many factors that may contribute to increased fracture risk in osteoporosis, diabetes, and aging, is the accumulation of advanced glycation endproducts (AGEs) in bone. AGEs are produced when chemical crosslinks formed between proteins or lipids and extracellular sugars [5-6,15,47];​ this can occur in bone tissue’s collagen matrix due to a natural biochemical reaction. It is generally accepted that AGEs increase with aging and weaken the mechanical properties of the bone. However, there are limited data in the literature and some of this data is contradicting, making it difficult to draw comparisons and conclusions about the effects of AGEs in bone. One discrepancy is in regard to whether there are higher levels of AGEs in cortical or trabecular bone, which are the two bone types that compose different regions of bone and play varying roles in bone’s mechanical behavior. Furthermore, we needed a clearer understanding of how the presence of measured AGEs affect mechanical behavior of trabecular and cortical bone via traditional mechanical tests and imaging. We hypothesized that bone samples in a high sugar environment would have weaker mechanical properties compared to control counter parts. We obtained cortical beams and trabecular cores from aging human cadaveric bone samples (female, ages:​ 50-100 years). Specimens were divided into non-incubated, vehicle-incubated, and ribose-incubated groups, where the ribose sugar incubation represents a high sugar diabetic environment. After incubation, specimens were imaged by microcomputed tomography, mechanically tested, and quantified for total fluorescent AGEs. In conclusion, there were no differences in AGEs between cortical and trabecular bone;​ however, there was a positive correlation between fracture toughness and age (r=0.59, p≤0.05) and a negative correlation between total strain energy and AGEs in ribose-incubated trabecular bone (r=-0.71, p≤0.05). There was not a statistically significant difference between AGEs and other compression testing variables such as pre- and post- yield strain, ultimate stress, strain, and toughness.