Current and emerging technologies for defining and validating tendon cell fate

Huang, Alice H.; Galloway, Jenna L.

Affiliations

¹Department of Orthopedic Surgery, Columbia University, New York, New York, USA

²Department of Orthopaedic Surgery, Center for Regenerative Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA

³Harvard Stem Cell Institute, Harvard University, Cambridge, Massachusetts, USA

Abstract and keywords

The tendon field has been flourishing in recent years with the advent of new tools and model systems. The recent ORS 2022 Tendon Section Conference brought together researchers from diverse disciplines and backgrounds, showcasing studies in biomechanics and tissue engineering to cell and developmental biology and using models from zebrafish and mouse to humans. This perspective aims to summarize progress in tendon research as it pertains to understanding and studying tendon cell fate. The successful integration of new technologies and approaches have the potential to further propel tendon research into a new renaissance of discovery. However, there are also limitations with the current methodologies that are important to consider when tackling research questions. Altogether, we will highlight recent advances and technologies and propose new avenues to explore tendon biology.

Keywords: mouse models; multiomics; sequencing; tendon development; tendon differentiation

Links

DOI: 10.1002/jor.25632

PubMed: 37211925

WoS: 001002616200001

History

Received: 2023-01-13

Revised: 2023-05-09

Accepted: 2023-05-18

Cited Works (7)

Year	Entry
2000	Kannus P. Structure of the tendon connective tissue. Scand J Med Sci Sports. December 2000;10(6):312-320.
1997	Otto F, Thornell AP, Crompton T, Denzel A, Gilmour KC, Rosewell IR, Stamp GW, Beddington RS, Mundlos S, Olsen BR, Selby PB, Owen MJ. Cbfa1, a candidate gene for cleidocranial dysplasia syndrome, is essential for osteoblast differentiation and bone development. Cell. May 30, 1997;89(5):765-771.
2023	Henke K, Farmer DT, Niu X, Kraus JM, Galloway JL, Youngstrom DW. Genetically engineered zebrafish as models of skeletal development and regeneration. Bone. February 2023;167:116611.
2007	Bi Y, Ehirchiou D, Kilts TM, Inkson CA, Embree MC, Sonoyama W, Li L, Leet AI, Seo B-M, Zhang L, Shi S, Young MF. Identification of tendon stem/progenitor cells and the role of the extracellular matrix in their niche. Nat Med. October 2007;13(10):1219-1227.
2002	Kalajzic I, Kalajzic Z, Kaliterna M, Gronowicz G, Clark SH, Lichtler AC, Rowe D. Use of type I collagen green fluorescent protein transgenes to identify subpopulations of cells at different stages of the osteoblast lineage. J Bone Miner Res. January 2002;17(1):15-25.
1997	Komori T, Yagi H, Nomura S, Yamaguchi A, Sasaki K, Deguchi K, Shimizu Y, Bronson RT, Gao Y-H, Inada M, Sato M, Okamoto R, Kitamura Y, Yoshiki S, Kishimoto T. Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts. Cell. May 30, 1997;89(5):755-764.
1999	Bi W, Deng JM, Zhang Z, Behringer RR, de Crombrugghe B. Sox9 is required for cartilage formation. Nat Genet. May 1999;22(1):85-89.

Cited By (1)

Year	Entry
2023	Dyment NA, Kuntz AF, Soslowsky LJ. Special issue: guiding the future of tendon research. J Orthop Res. October 2023;41(10):2081-2081.