Approximately 60% of young adults who present with chronic hip pain suffer from hip-related groin pain (HRGP). HRGP causes individuals to experience pain, dysfunction, and have a lower quality of life when compared to healthy individuals. The most severe symptoms in these individuals occur during activities that cause the hip to go through a large range of motion, such as a squat. Hip muscle activation during this task may be altered in these individuals, yet we know little about its role in the disease process and pain level in those with HRGP. Current treatment for these individuals includes surgical and nonsurgical interventions;​ however, the effects of both treatments are not effective, and individuals tend to experience symptoms again within 2 years. Understanding muscle activation during squatting tasks may help us to develop a more effective and long-term solution when treating these patients. Therefore, the purpose of this study was to examine bilateral hip muscle activity of the tensor fascia latae, rectus femoris, gluteus medius, and gluteus maximus during single- and double-legged squats in individuals with HRGP. Specific Aims 1 and 2 were to compare bilateral differences in hip muscle activation during the doublelegged and single-legged squats, respectively. Specific Aim 3 examined differences in muscle activation on the symptomatic side between the double-legged and single-legged squats. Methods:​ Seven individuals (2M/5F, age:​ 25.83±3.37yrs, BMI:​ 25.92±4.83kg/m²) with unilateral HRGP were recruited to participate. Following informed consent, surface EMG sensors were placed bilaterally on the four hip muscles. Individuals were asked to perform maximal voluntary isometric contractions (MVICs) for each muscle. Subsequently, two sets of five double-legged squats and five individual single-legged squats were performed. Average RMS value of each muscle (expressed as %MVIC) during the squats were determined. T-tests were performed to compare between the affected and healthy legs (Specific Aims 1 and 2) or between tasks (Specific Aim 3) when the data were normally distributed. Mann-Whitney U tests were performed when the assumptions of normality were violated. The alpha value for all statistical tests was 0.05. Results/Conclusions:​ No significant differences in hip muscle activation were found bilaterally during double- and single-legged squats for any of the muscles (p>0.05). However, when comparing the symptomatic side muscle activation between squat tasks, the gluteus maximus had higher activation during the single-legged squat (single-legged:​ 25.0 ± 13.5 %MVIC, double-legged:​ 12.6 ± 7.3 %MVIC, p<0.001). No other task differences were found. Synchronous data that were collected as part of a larger study revealed no differences in either hip muscle strength or squat depth between the symptomatic and asymptomatic sides. Thus, the lack of bilateral activation differences is not surprising. Due to the small subject pool, it is hard to determine concrete conclusions concerning activation of the hip musculature. Future studies are needed to better understand these findings in regard to hip pain and functional movement patterns in individuals with HRGP.