Kangfuxin accelerates extraction socket healing by promoting angiogenesis via upregulation of CCL2 in stem cells

Gao, Zheng-Rong<sup>1,*</sup>; Zhou, Ying-Hui<sup>1,2,*</sup>; Zhao, Ya-Qiong<sup>1</sup>; Zhao, Jie<sup>1</sup>; Ye, Qin<sup>1</sup>; Zhang, Shao-Hui<sup>1</sup>; Feng, Yao<sup>1</sup>; Tan, Li<sup>1</sup>; Liu, Qiong<sup>1</sup>; Chen, Yun<sup>1</sup>; Ouyang, Ze-Yue<sup>1</sup>; Hu, Jing<sup>1</sup>; Dusenge, Marie Aimee<sup>1</sup>; Feng, Yun-Zhi<sup>1</sup>; Guo, Yue<sup>1</sup>

Affiliations

¹Department of Stomatology, the Second Xiangya Hospital, Central South University, Changsha, China

²National Clinical Research Center for Metabolic Diseases, Hunan Provincial Key Laboratory of Metabolic Bone Diseases, and Department of Metabolism and Endocrinology, The Second Xiangya Hospital of Central South University, Changsha, China

Abstract and keywords

Kangfuxin (KFX) shows potential in wound healing, but its role in socket healing is unclear. This research finds increased bone mass, mineralization, and collagen deposition in KFX-treated mice. Mouse bone marrow mesenchymal stem cells, human periodontal ligament stem cells (hPDLSCs), and human dental pulp stem cells (hDPSCs) are treated with KFX under osteogenic induction. RNA-sequencing reveals upregulated chemokine-related genes, with a threefold increase in chemokine (C-C motif) ligand 2 (Ccl2). The conditioned medium (CM) of hPDLSCs and hDPSCs treated with KFX promotes endothelial cell migration and angiogenesis. Ccl2 knockdown abolishes CM-induced endothelial cell migration and angiogenesis, which can be reversed by recombinant CCL2 treatment. KFX-treated mice showed increased vasculature. In conclusion, KFX increases the expression of CCL2 in stem cells, promoting bone formation and mineralization in the extraction socket by inducing endothelial cell angiogenesis.

Keywords: KANGFUXIN; EXTRACTION SOCKET HEALING; STEM CELLS; CCL2; ANGIOGENESIS

Links

DOI: 10.1002/jbmr.4860

PubMed: 37221128

WoS: 001007634300001

History

Received: 2023-02-04

Revised: 2023-05-10

Accepted: 2023-05-18

Cited Works (1)

Year	Entry
2014	Kusumbe AP, Ramasamy SK, Adams RH. Coupling of angiogenesis and osteogenesis by a specific vessel subtype in bone. Nature. March 20, 2014;507(7492):323-328.