A method of measuring 3D strain in-vitro is presented. Regional 3D meniscal strain has not been studied in a large number of human knees under simulated physiological loading. Strain is calculated by tracking markers implanted within the meniscal tissue using computed tomography imaging under simulated physiologically relevant loading. No statistically significant difference between strain in the middle or posterior of the meniscus or between the principal directions of strain is uncovered.

Strain in meniscal allograft transplants (MAT) is also evaluated with two surgical fixation techniques;​ both use soft tissue anterior and posterior root fixation via transosseous suture but one also includes a third peripheral transosseous suture fixation. No significant difference was found. These results suggest that postoperative MAT perform in a similar manner to the native meniscus, and the addition of a peripheral anchor may not be necessary to replicate native tissue strain or improve the chondroprotective effect.