Trabecular bone score (TBS) derived from secondary analysis of lumbar spine dual-energy X-ray absorptiometry (DXA) scans improves fracture prediction independent of bone mineral density (BMD) in adults. The utility of TBS to assess fracture risk in younger patients has not been established because pediatric norms have been lacking. Robust TBS reference data from the Bone Mineral Density in Childhood Study (BMDCS) have been published. TBS values for the BMDCS study were derived using an algorithm that accounts for tissue thickness (TBS_TH) rather than the commercially available algorithm that adjusts for body mass index (BMI;​ TBS_BMI). We examined the magnitude of differences in TBS_TH and TBS_BMI in a cohort of 189 healthy youth. TBS values using both algorithms increased with age and pubertal development in a similar pattern. However, TBS_BMI values were systematically and significantly higher than TBS_TH (mean = 0.06, p < 0.0001). The difference between calculated TBS_BMI and TBS_TH was not uniform. Differences were greater at lower TBS values, in males, in older individuals, in those at later Tanner stages, and in those with a greater BMI Z-score. These systematic differences preclude the development of a simple formula to allow conversion of TBS_BMI to TBS_TH “equivalents.” Because of these systematic differences in these two algorithms, using an individual's TBS_BMI to calculate a Z-score using the BMDCS TBS_TH reference values results in a falsely higher TBS Z-score (differences mean = 0.73, interquartile range [IQR] = 0.3 to 1.6). Until TBS_TH software for Hologic DXA equipment becomes commercially available, BMDCS TBS reference norms should not be used.