Shear forces on the buttocks while seated are directly linked to friction, yet the frictional properties at the seat interface are unknown. Shear forces are one of the factors related to increase risk of pressure injury formation. The goals of this study included determining coefficients of friction between three cushion covers and two clothing fabrics using a mechanical system as well as human participants and to evaluate the impact of the cushion covers on shear loading on the buttocks while seated. A chair with separate seat pan tilt and back recline movements was built and instrumented with reflective markers and a load cell. A motion capture system and load cell were used to determine the angles of seat pan tilt at which the sled and participants started sliding, as well as shear forces at three recline angles for three cushion covers (vinyl, one-layer nylon, and two-layer nylon). Results showed the vinyl and two-layer nylon cushion covers respectively had the largest and smallest coefficients of friction for both pants materials. The coefficients of friction calculated with the human participants and rigid sled were within 10% of each other, demonstrating similar results. Further, increasing back recline increased shear load on the buttocks, while the two-layer nylon cover reduced shear forces seen on the buttocks. This work furthers the understanding of shear loading on the buttocks, will aid in the protocols for reducing pressure injuries, and suggests that coefficients of friction found using rigid bodies may be applied to deformable bodies.