Anterior Cruciate Ligament injuries are debilitating and have a time-consuming rehabilitation process. Females are 2 to 8 times more likely to sustain non-contact ACL injuries than men, particularly in sports that require frequent cutting, accelerating, jumping, and landing. Although there are many suggestions as to why this high incidence of injury occurs in women, there are no real explanations. The present study used a within subjects design to investigate the effect of functional fatigue on the Hoffmann’s Reflex in female Division I soccer players. The H-Reflex was used to test the action of the stretch reflex within the quadriceps to determine how human reflexes are affected due to fatigue. The onset, duration, and peak of H-reflex were measured for each subject before and after fatigue. A significant change in stimulus required to produce the onset of H-reflex was found when comparing pre and post fatigue results.