Infertility is a prevalent medical condition affecting an estimated 12% of women in the United States. In vitro fertilization (IVF) is the most common treatment for infertility; however IVF has a low success rate of approximately 30% and may cost upwards of $60,000. It is generally hypothesized that the low IVF success rate is due to insufficient binding between the blastocyst and uterine endometrium, a process called implantation. Blastocyst implantation occurs in three main steps: a primary L-selectin mediated adhesion, followed by a secondary integrin mediated adhesion, and lastly invasion into the endometrium. L-selectin binding strength, in leukocytes, is relatively weak, on the order of 1-10 dynes/cm² compared to integrin binding which may be greater than 100 dynes/cm². We hypothesize that surface L-selectin/sialyl Lewis X mechanics drive blastocyst adhesion and insufficiencies in these mechanics are in part responsible for low IVF success rates. Due to legal and ethical restrictions direct testing of blastocysts is difficult to perform. To alleviate these restrictions, this work presents a novel three-dimensional cellular model of blastocyst implantation for quantifying L-selectin mediated blastocyst/endometrium adhesion. This cellular model consists of three parts: a three-dimensional blastocyst mimic of Jeg-3 cells, or “trophosphere” expressing L-selectin; a model endometrium of Ishikawa cells expressing the L-selectin ligand, sialyl Lewis X, and a method of quantifying adhesion mechanics. Trophospheres were shown to detach from Ishikawa cells by 11x10⁻⁵ dyne of force and 2.1x10⁻⁶ dyne-cm of torque. This result is consistent with L-selectin adhesion mechanics in leukocytes and marks the first time L-selectin mediated blastocyst implantation mechanics have been quantified in any regard. These results also provide a framework for investigating the role of adhesion mechanics during blastocyst implantation and may aid in the development of new IVF techniques and infertility treatments in general.