An in vitro model of neural trauma: device characterization and calcium response to mechanical stretch

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Geddes, Donna M.¹; Cargill, II, Robert S.¹

An in vitro model of neural trauma: device characterization and calcium response to mechanical stretch

J Biomech Eng. June 2001;123(3):247-255

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Affiliations

¹School of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA 30332-0405
Abstract and keywords

An in vitro model for neural trauma was characterized and validated. The model is based on a novel device that is capable of applying high strain rate, homogeneous, and equibiaxial deformation to neural cells in culture. The deformation waveform is fully arbitrary and controlled via closed-loop feedback. Intracellular calcium ([Ca²⁺]_i) alterations were recorded in real time throughout the imposed strain with an epifluorescent microscopy system. Peak change in [Ca²⁺]_i, recovery of [Ca²⁺]_i, and percent responding NG108-15 cells were shown to be dependent on strain rate (1−1 to 10−1) and magnitude (0.1 to 0.3 Green’s Strain). These measures were also shown to depend significantly on the interaction between strain rate and magnitude. This model for neural trauma is a robust system that can be used to investigate the cellular tolerance and response to traumatic brain injury.

Keywords:
Links

DOI: 10.1115/1.1374201

PubMed: 11476368

WoS: 000169878700007
History

Received: 1999-12-27

Revised: 2001-01-11

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Cited By (24)

Year	Entry
2012	Rashid B, Destrade M, Gilchrist MD. Experimental characterisation of neural tissue at collision speeds. In: Proceedings of the 2012 International IRCOBI Conference on the Biomechanics of Injury. September 12-14, 2012; Dublin, Ireland.405-416.
2003	Morrison B III, Cater HL, Wang CC-B, Thomas FC, Hung CT, Ateshian GA, Sundstrom LE. A tissue level tolerance criterion for living brain developed with an in vitro model of traumatic mechanical loading. Stapp Car Crash J. 2003;47:93-105. SAE 2003-22-0006.
2007	Tamura A, Nagayama K, Matsumoto T, Hayashi S. Variation in nerve fiber strain in brain tissue subjected to uniaxial stretch. Stapp Car Crash J. 2007;51:139-154. SAE 2007-22-0006.
2009	Fijalkowski RJ, Yoganandan N, Zhang J, Pintar FA. A finite element model of region-specific response for mild diffuse brain injury. Stapp Car Crash J. 2009;53:193-213. SAE 2009-22-0007.
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2005	LaPlaca MC, Cullen DK, McLoughlin JJ, Cargill RS II. High rate shear strain of three-dimensional neural cell cultures: a new in vitro traumatic brain injury model. J Biomech. May 2005;38(5):1093-1105.
2006	Cater HL, Sundstrom LE, Morrison B III. Temporal development of hippocampal cell death is dependent on tissue strain but not strain rate. J Biomech. 2006;39(16):2810-2818.
2006	Morrison B III, Cater HL, Benham CD, Sundstrom LE. An in vitro model of traumatic brain injury utilising two-dimensional stretch of organotypic hippocampal slice cultures. J Neurosci Meth. 2006;150(2):192-201.
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2015	Shoemaker JT. Development of an in Vitro Model of Neuroinflammation for Studying Secondary Injury Mechanisms in Traumatic Brain Injury [PhD thesis]. Atlanta, GA: Georgia Institute of Technology; August 2015.
2014	Patton DA. The Biomechanical Determinants of Sports-Related Concussion: Finite Element Simulations of Unhelmeted Head Impacts to Evaluate Kinematic and Tissue-Level Predictors of Injury and Investigate the Design Implications for Soft-Shell Headgear [PhD thesis]. University of New South Wales; March 2014.
2002	Lusardi TAG. Initiation and Propagation of Mechanically Induced Neuronal Injury [PhD thesis]. Philadelphia, PA: University of Pennsylvania; 2002.
2018	Hlavac NM. Attributes of Astrocyte Response to Mechano-Stimulation by High-Rate Overpressure [PhD thesis]. Virginia Polytechnic Institute and State University; October 25, 2018.
2020	Zhou R. Development of Rat Head Finite Element Model and Tissue Level Biomechanical Threshold for Traumatic Axonal Injury [PhD thesis]. Detroit, MI: Wayne State University; May 2020.