Interaction of water with native collagen

Nomura, S.<sup>1,2</sup>; Hiltner, A.<sup>1</sup>; Lando, J. B.<sup>1</sup>; Baer, E.<sup>1</sup>

Affiliations

¹Department of Macromolecular Science, Case Western Reserve University, Cleveland, Ohio 44106

²Present address: Department of Polymer Science, Faculty of Engineering, Kyoto University, Kyoto 606 Japan

Abstract

The interaction of water with collagenous tissue was investigated using dynamic mechanical spectroscopy and cryogenic X‐ray techniques. The loss spectrum was found to be very sensitive to water which is highly associated with the macromolecule. Two water‐sensitive loss peaks were observed below 0°C: the β₂ or “water dispersion” at 150°K and the β₁ at 200°K which is attributed to motion of polar side chains. Changes in peak temperature and intensity were not continuous with water content, but exhibited regimes in behavior which were associated with two types of nonfreezable water, structural and bound water. In cryogenic X‐ray experiments, specimens which contained some freezable water exhibited reflections identified with the cubic form of ice. These ice crystals underwent an irreversible transition to the more common hexagonal form when warmed above 200°K. On the basis of these experiments, a model for the hydration of native collagenous tissue was proposed.

Links

DOI: 10.1002/bip.1977.360160202

PubMed: 831859

WoS: A1977CW90800001

History

Received: 1976-04-19

Accepted: 1976-06-08

Cited By (25)

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