Teriparatide improves microarchitectural characteristics of peri-implant bone in orchiectomized rats

Gomes-Ferreira, P. H. S.<sup>1</sup>; de Oliveira, D.<sup>2</sup>; Frigério, P. B.<sup>2</sup>; de Souza Batista, F. R.<sup>1</sup>; Grandfield, K.<sup>3</sup>; Okamoto, R.<sup>2,4</sup>

Affiliations

¹Department of Surgery and Integrated Clinic, Araçatuba Dental School, São Paulo State University, Aracatuba, Brazil

²Department of Basic Sciences, Araçatuba Dental School, São Paulo State University, Araçatuba, Brazil

³Department of Materials Science and Engineering, McMaster University, Hamilton, Canada

⁴Research Productivity Scholarship (Process: 306389/2017-7), Aracatuba, Brazil

Abstract and keywords

Summary: This study evaluated the peri-implant bone repair in orchiectomized rats receiving intermittently PTH 1-34. The treatment returned the bone quality and quantity of the animals to normal in the computerized microtomography, laser confocal microscopy, and histological analysis. The PTH 1-34 promoted marked bone formation with increased volume, improved quality, and greater bone turnover.

Introduction: Osteoporosis can be a problem in implant osseointegration. So this study aimed to evaluate the quantity and quality of peri-implant bone repair in orchiectomized Wistar rats receiving intermittently administered PTH 1-34.

Methods: Animals (n = 24) were divided into 3 groups: healthy control (SHAM), orchiectomized (ORQ), and orchiectomized and treated with 0.5 μg/kg/day PTH 1-34 (TERI), and each received an implant in the right and left tibial metaphysis, which was allowed to repair for 60 days. The resultant bone formation was evaluated through computerized microtomography (micro-CT) to compare the percent bone volume (BV/TV), trabecular thickness (Tb.Th), trabecular number and separation (Tb.N, Tb.Sp), and bone implant contact (BIC) through the intersection surface (i.S) between groups. Laser confocal microscopy was used to evaluate fluorochrome areas for mineral apposition rate (MAR) and neoformed bone area (NBA). In addition, histological evaluation of calcified tissues with Stevenel blue and alizarin red staining was performed.

Results: Treatment with PTH 1-34 returned the bone quality and quantity of the osteoporotic animal to normal, as the TERI group presented statistically significant higher values for BV/TV, Tb.Th, and BIC parameters compared with ORQ (p < 0.05), but when compared with SHAM (p > 0.05), no statistical difference was noted. In addition, in the bone turnover analysis (MAR, NBA) for TERI, the highest results are presented, followed by SHAM, and then ORQ (TERI × ORQ: p < 0.05).

Conclusions: Intermittent treatment with PTH 1-34 on orchiectomized animals promoted marked bone formation with increased volume, improved quality, and greater bone turnover in the peri-implant space, returning the bone quality and quantity to the present standard in healthy animals.

Keywords: Bone histomorphometry; Bone QCT; Implants; Micro-computed tomography; Osseointegration; Osteoporosis; PTH

Links

DOI: 10.1007/s00198-020-05431-y

PubMed: 32383065

WoS: 000530997000001

History

Received: 2020-01-13

Accepted: 2020-04-22

Online: 2020-05-07

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Cited By (1)

Year	Entry
2021	Romanowicz GE. The Role of Collagen Cross-Linking in Craniofacial and Long Bone Mineralization and Healing [PhD thesis]. University of Michigan; 2021.