Percentage finger systolic blood pressure (%FSBP) in response to finger cooling is used to assess vascular components of the hand-arm vibration syndrome and the measurement method is under discussion for standardization. It has been suggested that measurement circumstances including room temperature may influence %FSBP. We investigated the influence of room temperature on %FSBP response to finger cooling in healthy subjects. Six healthy male subjects who were medical students volunteered for the study. Multi-Channel Plethysmograph was used for simultaneous multi-finger FSBP measurements. The examination room was kept at 21 ± 1 °C and 25 ± 1 °C, and the subjects were randomly assigned. %FSBPs for the index, middle, ring and little fingers at 15 °C and 10 °C cuff-water temperatures were calculated. Four-way analysis of variance was performed to determine the independent influence of the subject factor, room temperature factor, finger factor, and cuff-water temperature factor on %FSBP. The room temperature as an independent factor was significant (p<0.01). The results also indicated that the temperature of 21 ± 1 °C may be better than that of 25 ± 1 °C for room temperature during cold provocation test measuring FSBP. Room temperature might influence %FSBP response to finger cooling, and therefore, is expected to be controlled at 21 ± 1 °C when assessing peripheral vascular components using %FSBP.