This study aims to compare autonomic nervous activity observed during cold-stress test using different hand covering methods during water immersion as follows;​ polyethylene gloves, natural rubber gloves, and bare hands. Six normal healthy male students volunteered to participate in the test through which electrocardiogram was recorded. After recording baseline data for 5 min, subjects were to put on specified gloves or nothing according to the protocol and immersed both hands into stirred water at 12 °C for 5 min. The subjects removed their hands from the water to discard the gloves and kept at rest for 20 min of recovery thereafter. Then, heart rate variability was analyzed as autonomic nervous system indexes for each 5 min during pre-immersion, hand immersion, and recovery time. Significant differences in high frequency (0.15-0.40 Hz) component power were found in case of bare hands and natural rubber glove use (p<0.05, respectively). Low frequency (0.04-0.15 Hz) to high frequency component power ratio was changed significantly only in case of bare hands (p<0.05). Our results support that covering methods during hand immersion influenced heart rate variability in healthy subjects and indicated that hand immersion into cold water may influence both sympathetic and parasympathetic nervous activity.